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1wsd

From Proteopedia

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{{STRUCTURE_1wsd| PDB=1wsd | SCENE= }}
{{STRUCTURE_1wsd| PDB=1wsd | SCENE= }}
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'''Alkaline M-protease form I crystal strcuture'''
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===Alkaline M-protease form I crystal strcuture===
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==Overview==
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M-protease is a subtilisin-family serine protease produced by an alkaliphilic Bacillus sp. strain. Optimal enzymatic activity of the protein occurs at pH 12.3. The crystal structure of M-protease (space group P2(1)2(1)2(1), a = 62.3, b = 75.5, c = 47.2 A) has been refined to a crystallographic R-factor of 17.2% at 1.5 A resolution. The alkaline adaptation mechanism of the enzyme was analyzed. Molecular phylogeny construction was used to determine the amino acid substitutions that occurred during the high-alkaline adaptation process. This analysis revealed a decrease in the number of negatively charged amino acids (aspartic acid and glutamic acid) and lysine residues and an increase in arginine and neutral hydrophilic amino acids (histidine, asparagine and glutamine) residues during the course of adaptation. These substitutions increased the isoelectric point of M-protease. Some of the acquired arginine residues form hydrogen bonds or ion pairs to combine both N- and C-terminal regions of M-protease. The substituted residues are localized to a hemisphere of the globular protein molecule where positional shifts of peptide segments, relative to those of the less alkaliphilic subtilisin Carlsberg, are observed. The biased distribution and interactions caused by the substituted residues seem to be responsible for stabilization of the conformation in a high-alkaline condition.
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{{ABSTRACT_PUBMED_9278275}}
==About this Structure==
==About this Structure==
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[[Category: High-alkaline]]
[[Category: High-alkaline]]
[[Category: Subtilisin]]
[[Category: Subtilisin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:01:06 2008''

Revision as of 05:01, 29 July 2008

Image:1wsd.png

Template:STRUCTURE 1wsd

Alkaline M-protease form I crystal strcuture

Template:ABSTRACT PUBMED 9278275

About this Structure

1WSD is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structure of M-protease: phylogeny aided analysis of the high-alkaline adaptation mechanism., Shirai T, Suzuki A, Yamane T, Ashida T, Kobayashi T, Hitomi J, Ito S, Protein Eng. 1997 Jun;10(6):627-34. PMID:9278275

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