2owc

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{{STRUCTURE_2owc| PDB=2owc | SCENE= }}
{{STRUCTURE_2owc| PDB=2owc | SCENE= }}
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'''Structure of a covalent intermediate in Thermus thermophilus amylomaltase'''
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===Structure of a covalent intermediate in Thermus thermophilus amylomaltase===
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==Overview==
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Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book movie of the amylomaltase catalytic cycle in atomic detail. The structures include a covalent glycosyl enzyme intermediate and a covalent intermediate in complex with an analogue of a co-substrate and show how the structures of both enzyme and substrate respond to the changes required by the catalytic cycle as it proceeds. Notably, the catalytic nucleophile changes conformation dramatically during the reaction. Also, Gln-256 on the 250s loop is involved in orienting the substrate in the +1 site. The absence of a suitable base in the covalent intermediate structure explains the low hydrolysis activity.
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(as it appears on PubMed at http://www.pubmed.gov), where 17420245 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17420245}}
==About this Structure==
==About this Structure==
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[[Category: Beta-alpha-barrel]]
[[Category: Beta-alpha-barrel]]
[[Category: Glycosyl-enzyme]]
[[Category: Glycosyl-enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:47:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:04:56 2008''

Revision as of 05:05, 29 July 2008

Image:2owc.png

Template:STRUCTURE 2owc

Structure of a covalent intermediate in Thermus thermophilus amylomaltase

Template:ABSTRACT PUBMED 17420245

About this Structure

2OWC is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase., Barends TR, Bultema JB, Kaper T, van der Maarel MJ, Dijkhuizen L, Dijkstra BW, J Biol Chem. 2007 Jun 8;282(23):17242-9. Epub 2007 Apr 9. PMID:17420245

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