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1mvm

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[[Image:1mvm.gif|left|200px]]
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{{STRUCTURE_1mvm| PDB=1mvm | SCENE= }}
{{STRUCTURE_1mvm| PDB=1mvm | SCENE= }}
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'''MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C'''
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===MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C===
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==Overview==
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The three-dimensional crystal structure of the single-stranded DNA-containing ('full') parvovirus, minute virus of mice (MVM), has been determined to 3.5 A resolution. Both full and empty particles of MVM were crystallized in the monoclinic space group C2 with cell dimensions of a = 448.7, b = 416.7, c = 305.3 A and beta = 95.8 degrees. Diffraction data were collected at the Cornell High Energy Synchrotron Source using an oscillation camera. The crystals have a pseudo higher R32 space group in which the particles are situated at two special positions with 32 point symmetry, separated by (1/2)c in the hexagonal setting. The self-rotation function showed that the particles are rotated with respect to each other by 60 degrees around the pseudo threefold axis. Subsequently, a more detailed analysis of the structure amplitudes demonstrated that the correct space-group symmetry is C2 as given above. Only one of the three twofold axes perpendicular to the threefold axis in the pseudo R32 space group is a 'true' crystallographic twofold axis; the other two are only 'local' non-crystallographic symmetry axes. The known canine parvovirus (CPV) structure was used as a phasing model to initiate real-space electron-density averaging phase improvement. The electron density was easily interpretable and clearly showed the amino-acid differences between MVM and CPV, although the final overall correlation coefficient was only 0.63. The structure of MVM has a large amount of icosahedrally ordered DNA, amounting to 22% of the viral genome, which is significantly more than that found in CPV.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299974 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299974}}
==About this Structure==
==About this Structure==
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[[Category: Icosahedral virus]]
[[Category: Icosahedral virus]]
[[Category: Viral coat protein/nucleic acid]]
[[Category: Viral coat protein/nucleic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:46:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:29:52 2008''

Revision as of 06:29, 29 July 2008

Image:1mvm.png

Template:STRUCTURE 1mvm

MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C

Template:ABSTRACT PUBMED 15299974

About this Structure

1MVM is a Single protein structure of sequence from Minute virus of mice. Full crystallographic information is available from OCA.

Reference

Structure determination of minute virus of mice., Llamas-Saiz AL, Agbandje-McKenna M, Wikoff WR, Bratton J, Tattersall P, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):93-102. PMID:15299974

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