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2evq

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{{STRUCTURE_2evq| PDB=2evq | SCENE= }}
{{STRUCTURE_2evq| PDB=2evq | SCENE= }}
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'''Solution structure of HP7, a 12-residue beta hairpin'''
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===Solution structure of HP7, a 12-residue beta hairpin===
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==Overview==
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Minimized beta hairpins have provided additional data on the geometric preferences of Trp interactions in TW-loop-WT motifs. This motif imparts significant fold stability to peptides as short as 8 residues. High-resolution NMR structures of a 16- (KKWTWNPATGKWTWQE, DeltaG(U)(298) &gt;or= +7 kJ/mol) and 12-residue (KTWNPATGKWTE, DeltaG(U)(298) = +5.05 kJ/mol) hairpin reveal a common turn geometry and edge-to-face (EtF) packing motif and a cation-pi interaction between Lys(1) and the Trp residue nearest the C-terminus. The magnitude of a CD exciton couplet (due to the two Trp residues) and the chemical shifts of a Trp Hepsilon3 site (shifted upfield by 2.4 ppm due to the EtF stacking geometry) provided near-identical measures of folding. CD melts of representative peptides with the -TW-loop-WT- motif provided the thermodynamic parameters for folding, which reflect enthalpically driven folding at laboratory temperatures with a small DeltaC(p) for unfolding (+420 J K(-)(1)/mol). In the case of Asx-Pro-Xaa-Thr-Gly-Xaa loops, mutations established that the two most important residues in this class of direction-reversing loops are Asx and Gly: mutation to alanine is destabilizing by about 6 and 2 kJ/mol, respectively. All indicators of structuring are retained in a minimized 8-residue construct (Ac-WNPATGKW-NH(2)) with the fold stability reduced to DeltaG(U)(278) = -0.7 kJ/mol. NMR and CD comparisons indicate that -TWXNGKWT- (X = S, I) sequences also form the same hairpin-stabilizing W/W interaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 16669679 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16669679}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVQ OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVQ OCA].
==Reference==
==Reference==
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[[Category: Peptide]]
[[Category: Peptide]]
[[Category: Trp/trp packing]]
[[Category: Trp/trp packing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:10:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:33:41 2008''

Revision as of 06:33, 29 July 2008

Image:2evq.png

Template:STRUCTURE 2evq

Solution structure of HP7, a 12-residue beta hairpin

Template:ABSTRACT PUBMED 16669679

About this Structure

Full experimental information is available from OCA.

Reference

Minimization and optimization of designed beta-hairpin folds., Andersen NH, Olsen KA, Fesinmeyer RM, Tan X, Hudson FM, Eidenschink LA, Farazi SR, J Am Chem Soc. 2006 May 10;128(18):6101-10. PMID:16669679

Page seeded by OCA on Tue Jul 29 09:33:41 2008

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