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1wy4

From Proteopedia

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[[Image:1wy4.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1wy4| PDB=1wy4 | SCENE= }}
{{STRUCTURE_1wy4| PDB=1wy4 | SCENE= }}
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'''Chicken villin subdomain HP-35, K65(NLE), N68H, pH5.1'''
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===Chicken villin subdomain HP-35, K65(NLE), N68H, pH5.1===
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==Overview==
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The 35-residue subdomain of the villin headpiece (HP35) is a small ultrafast folding protein that is being intensely studied by experiments, theory, and simulations. We have solved the x-ray structures of HP35 and its fastest folding mutant [K24 norleucine (nL)] to atomic resolution and compared their experimentally measured folding kinetics by using laser temperature jump. The structures, which are in different space groups, are almost identical to each other but differ significantly from previously solved NMR structures. Hence, the differences between the x-ray and NMR structures are probably not caused by lattice contacts or crystal/solution differences, but reflect the higher accuracy of the x-ray structures. The x-ray structures reveal important details of packing of the hydrophobic core and some additional features, such as cross-helical H bonds. Comparison of the x-ray structures indicates that the nL substitution produces only local perturbations. Consequently, the finding that the small stabilization by the mutation is completely reflected in an increased folding rate suggests that this region of the protein is as structured in the transition state as in the folded structure. It is therefore a target for engineering to increase the folding rate of the subdomain from approximately 0.5 micros(-1) for the nL mutant to the estimated theoretical speed limit of approximately 3 micros(-1).
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The line below this paragraph, {{ABSTRACT_PUBMED_15894611}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15894611 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15894611}}
==About this Structure==
==About this Structure==
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[[Category: Kubelka, J.]]
[[Category: Kubelka, J.]]
[[Category: Villin headgroup subdomain]]
[[Category: Villin headgroup subdomain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:17:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:42:27 2008''

Revision as of 06:42, 29 July 2008

Image:1wy4.png

Template:STRUCTURE 1wy4

Chicken villin subdomain HP-35, K65(NLE), N68H, pH5.1

Template:ABSTRACT PUBMED 15894611

About this Structure

1WY4 is a Single protein structure. Full crystallographic information is available from OCA.

Reference

High-resolution x-ray crystal structures of the villin headpiece subdomain, an ultrafast folding protein., Chiu TK, Kubelka J, Herbst-Irmer R, Eaton WA, Hofrichter J, Davies DR, Proc Natl Acad Sci U S A. 2005 May 24;102(21):7517-22. Epub 2005 May 13. PMID:15894611

Page seeded by OCA on Tue Jul 29 09:42:27 2008

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