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1nop

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[[Image:1nop.gif|left|200px]]
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{{STRUCTURE_1nop| PDB=1nop | SCENE= }}
{{STRUCTURE_1nop| PDB=1nop | SCENE= }}
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'''Crystal structure of human tyrosyl-DNA phosphodiesterase (Tdp1) in complex with vanadate, DNA and a human topoisomerase I-derived peptide'''
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===Crystal structure of human tyrosyl-DNA phosphodiesterase (Tdp1) in complex with vanadate, DNA and a human topoisomerase I-derived peptide===
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==Overview==
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Tyrosyl-DNA phosphodiesterase (Tdp1) is a member of the phospholipase D superfamily and acts as a DNA repair enzyme that removes stalled topoisomerase I- DNA complexes by hydrolyzing the bond between a tyrosine side chain and a DNA 3' phosphate. Despite the complexity of the substrate of this phosphodiesterase, vanadate succeeded in linking human Tdp1, a tyrosine-containing peptide, and a single-stranded DNA oligonucleotide into a quaternary complex that mimics the transition state for the first step of the catalytic reaction. The conformation of the bound substrate mimic gives compelling evidence that the topoisomerase I-DNA complex must undergo extensive modification prior to cleavage by Tdp1. The structure also illustrates that the use of vanadate as the central moiety in high-order complexes has the potential to be a general method for capturing protein-substrate interactions for phosphoryl transfer enzymes, even when the substrates are large, complicated, and unusual.
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(as it appears on PubMed at http://www.pubmed.gov), where 12618186 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12618186}}
==About this Structure==
==About this Structure==
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[[Category: Transition state mimic]]
[[Category: Transition state mimic]]
[[Category: Vanadate complex]]
[[Category: Vanadate complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:47:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:16:14 2008''

Revision as of 07:16, 29 July 2008

Image:1nop.png

Template:STRUCTURE 1nop

Crystal structure of human tyrosyl-DNA phosphodiesterase (Tdp1) in complex with vanadate, DNA and a human topoisomerase I-derived peptide

Template:ABSTRACT PUBMED 12618186

About this Structure

1NOP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of a transition state mimic for Tdp1 assembled from vanadate, DNA, and a topoisomerase I-derived peptide., Davies DR, Interthal H, Champoux JJ, Hol WG, Chem Biol. 2003 Feb;10(2):139-47. PMID:12618186

Page seeded by OCA on Tue Jul 29 10:16:14 2008

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