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1ulm

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{{STRUCTURE_1ulm| PDB=1ulm | SCENE= }}
{{STRUCTURE_1ulm| PDB=1ulm | SCENE= }}
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'''Crystal Structure of Pokeweed Lectin-D2 complexed with tri-N-acetylchitotriose'''
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===Crystal Structure of Pokeweed Lectin-D2 complexed with tri-N-acetylchitotriose===
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==Overview==
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The roots of pokeweed (Phytolacca americana) are known to contain the lectins designated PL-A, PL-B, PL-C, PL-D1, and PL-D2. Of these lectins, the crystal structures of two PLs, the ligand-free PL-C and the complex of PL-D2 with tri-N-acetylchitotriose, have been determined at 1.8A resolution. The polypeptide chains of PL-C and PL-D2 form three and two repetitive chitin-binding domains, respectively. In the crystal structure of the PL-D2 complex, one trisaccharide molecule is shared mainly between two neighboring molecules related to each other by a crystallographic 2(1)-screw axis, and infinite helical chains of complexed molecules are generated by the sharing of ligand molecules. The crystal structure of PL-C reveals that the molecule is a dimer of two identical subunits, whose polypeptide chains are located in a head-to-tail fashion by a molecular 2-fold axis. Three putative carbohydrate-binding sites in each subunit are located in the dimer interface. The dimerization of PL-C is performed through the hydrophobic interactions between the carbohydrate-binding sites of the opposite domains in the dimer, leading to a distinct dimerization mode from that of wheat-germ agglutinin. Three aromatic residues in each carbohydrate-binding site of PL-C are involved in the dimerization. These residues correspond to the residues that interact mainly with the trisaccharide in the PL-D2 complex and appear to mimic the saccharide residues in the complex. Consequently, the present structure of the PL-C dimer has no room for accommodating carbohydrate. The quaternary structure of PL-C formed through these putative carbohydrate-binding residues may lead to the lack of hemagglutinating activity.
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==About this Structure==
==About this Structure==
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[[Category: Hevein domain]]
[[Category: Hevein domain]]
[[Category: Lectin]]
[[Category: Lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:59:02 2008''

Revision as of 07:59, 29 July 2008

Image:1ulm.png

Template:STRUCTURE 1ulm

Crystal Structure of Pokeweed Lectin-D2 complexed with tri-N-acetylchitotriose

Template:ABSTRACT PUBMED 14623194

About this Structure

Full crystallographic information is available from OCA.

Reference

Similarity between protein-protein and protein-carbohydrate interactions, revealed by two crystal structures of lectins from the roots of pokeweed., Hayashida M, Fujii T, Hamasu M, Ishiguro M, Hata Y, J Mol Biol. 2003 Nov 28;334(3):551-65. PMID:14623194

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