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2j6g

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[[Image:2j6g.gif|left|200px]]
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{{STRUCTURE_2j6g| PDB=2j6g | SCENE= }}
{{STRUCTURE_2j6g| PDB=2j6g | SCENE= }}
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'''FAEG FROM F4AC ETEC STRAIN 5_95, PRODUCED IN TOBACCO PLANT CHLOROPLAST'''
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===FAEG FROM F4AC ETEC STRAIN 5_95, PRODUCED IN TOBACCO PLANT CHLOROPLAST===
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==Overview==
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F4 fimbriae encoded by the fae operon are the major colonization factors associated with porcine neonatal and postweaning diarrhoea caused by enterotoxigenic Escherichia coli (ETEC). Via the chaperone/usher pathway, the F4 fimbriae are assembled as long polymers of the major subunit FaeG, which also possesses the adhesive properties of the fimbriae. Intrinsically, the incomplete fold of fimbrial subunits renders them unstable and susceptible to aggregation and/or proteolytic degradation in the absence of a specific periplasmic chaperone. In order to test the possibility of producing FaeG in plants, FaeG expression was studied in transgenic tobacco plants. FaeG was directed to different subcellular compartments by specific targeting signals. Targeting of FaeG to the chloroplast results in much higher yields than FaeG targeting to the endoplasmic reticulum or the apoplast. Two chloroplast-targeted FaeG variants were purified from tobacco plants and crystallized. The crystal structures show that chloroplasts circumvent the absence of the fimbrial assembly machinery by assembling FaeG into strand-swapped dimers. Furthermore, the structures reveal how FaeG combines the structural requirements of a major fimbrial subunit with its adhesive role by grafting an additional domain on its Ig-like core.
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{{ABSTRACT_PUBMED_17368480}}
==About this Structure==
==About this Structure==
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[[Category: Ig-fold]]
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[[Category: Strand swapping]]
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Revision as of 08:02, 29 July 2008

Image:2j6g.png

Template:STRUCTURE 2j6g

FAEG FROM F4AC ETEC STRAIN 5_95, PRODUCED IN TOBACCO PLANT CHLOROPLAST

Template:ABSTRACT PUBMED 17368480

About this Structure

2J6G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Chloroplasts assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers., Van Molle I, Joensuu JJ, Buts L, Panjikar S, Kotiaho M, Bouckaert J, Wyns L, Niklander-Teeri V, De Greve H, J Mol Biol. 2007 May 4;368(3):791-9. Epub 2007 Feb 22. PMID:17368480

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