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1zok

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[[Image:1zok.gif|left|200px]]
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{{STRUCTURE_1zok| PDB=1zok | SCENE= }}
{{STRUCTURE_1zok| PDB=1zok | SCENE= }}
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'''PDZ1 Domain Of Synapse Associated Protein 97'''
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===PDZ1 Domain Of Synapse Associated Protein 97===
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==Overview==
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The synapse-associated protein-97 (SAP97) is important in the proper trafficking and cell surface maintenance of the N-methyl-D-aspartate ionotropic glutamate receptor. The molecular scaffold/receptor interaction is mediated by the association of the C terminus of the NR2B subunit of the N-methyl-D-aspartate receptor with the PDZ domains of SAP97. Here, we characterize the binding of the C terminus of NR2B with the PDZ domains of SAP97 and determine the structure of the PDZ1-NR2B complex employing high-resolution NMR. Based on fluorescence anisotropy, the NR2B subunit binds to the first and second PDZ domains of SAP97, with higher affinity for PDZ2; no appreciable binding to PDZ3 could be measured. The structural features of the NR2B bound to PDZ1 is consistent with the canonical PDZ-binding motif with the glutamic acid at the -3 position of the C terminus (i.e. -E-S-D-V) interacting with the beta2/beta3 loop. Two sites within the loop of PDZ1 were replaced with the corresponding residue from PDZ2, D243G and P245Q. The former mutation, designed to remove a possible Coulombic repulsion between E(-3)(NR2B) and Asp-243 (PDZ1) has only a minimal effect on binding. The P245Q mutation leads to a 2-fold increase in binding affinity of NR2B, approaching that observed for wild-type PDZ2. These results indicate that modification of the beta2/beta3 loop provides an avenue for regulating the ligand specificity of PDZ domains.
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{{ABSTRACT_PUBMED_15929985}}
==About this Structure==
==About this Structure==
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1ZOK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZOK OCA].
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1ZOK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZOK OCA].
==Reference==
==Reference==
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[[Category: Sap97]]
[[Category: Sap97]]
[[Category: Synapse associated protein 97]]
[[Category: Synapse associated protein 97]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:52:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:06:04 2008''

Revision as of 08:06, 29 July 2008

Image:1zok.png

Template:STRUCTURE 1zok

PDZ1 Domain Of Synapse Associated Protein 97

Template:ABSTRACT PUBMED 15929985

About this Structure

1ZOK is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Structural characterization of the intermolecular interactions of synapse-associated protein-97 with the NR2B subunit of N-methyl-D-aspartate receptors., Wang L, Piserchio A, Mierke DF, J Biol Chem. 2005 Jul 22;280(29):26992-6. Epub 2005 Jun 1. PMID:15929985

Page seeded by OCA on Tue Jul 29 11:06:04 2008

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