1t8f

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{{STRUCTURE_1t8f| PDB=1t8f | SCENE= }}
{{STRUCTURE_1t8f| PDB=1t8f | SCENE= }}
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'''Crystal structure of phage T4 lysozyme mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A'''
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===Crystal structure of phage T4 lysozyme mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A===
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==Overview==
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In general, alpha-helical conformations in proteins depend in large part on the amino acid residues within the helix and their proximal interactions. For example, an alanine residue has a high propensity to adopt an alpha-helical conformation, whereas that of a glycine residue is low. The sequence preferences for beta-sheet formation are less obvious. To identify the factors that influence beta-sheet conformation, a series of scanning polyalanine mutations were made within the strands and associated turns of the beta-sheet region in T4 lysozyme. For each construct the stability of the folded protein was reduced substantially, consistent with removal of native packing interactions. However, the crystal structures showed that each of the mutants retained the beta-sheet conformation. These results suggest that the structure of the beta-sheet region of T4 lysozyme is maintained to a substantial extent by tertiary interactions with the surrounding parts of the protein. Such tertiary interactions may be important in determining the structures of beta-sheets in general.
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{{ABSTRACT_PUBMED_15340171}}
==About this Structure==
==About this Structure==
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[[Category: Poly-alanine mutation]]
[[Category: Poly-alanine mutation]]
[[Category: T4]]
[[Category: T4]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:39:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:36:05 2008''

Revision as of 08:36, 29 July 2008

Image:1t8f.png

Template:STRUCTURE 1t8f

Crystal structure of phage T4 lysozyme mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A

Template:ABSTRACT PUBMED 15340171

About this Structure

1T8F is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Alanine-scanning mutagenesis of the beta-sheet region of phage T4 lysozyme suggests that tertiary context has a dominant effect on beta-sheet formation., He MM, Wood ZA, Baase WA, Xiao H, Matthews BW, Protein Sci. 2004 Oct;13(10):2716-24. Epub 2004 Aug 31. PMID:15340171

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