1vah

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[[Image:1vah.gif|left|200px]]
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{{STRUCTURE_1vah| PDB=1vah | SCENE= }}
{{STRUCTURE_1vah| PDB=1vah | SCENE= }}
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'''Crystal structure of the pig pancreatic-amylase complexed with r-nitrophenyl-a-D-maltoside'''
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===Crystal structure of the pig pancreatic-amylase complexed with r-nitrophenyl-a-D-maltoside===
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==Overview==
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The X-ray structure analysis of a crystal of pig pancreatic alpha-amylase soaked with a rho-nitrophenyl-alpha-D-maltoside (pNPG2) substrate showed a pattern of electron density corresponding to the binding of a rho-nitrophenol unit at subsite -2 of the active site. Binding of the product to subsite -2 after hydrolysis of the pNPG2 molecules, may explain the low catalytic efficiency of the hydrolysis of pNPG2 by PPA. Except a small movement of the segment from residues 304-305 the typical conformational changes of the "flexible loop" (303-309), that constitutes the surface edge of the substrate binding cleft, were not observed in the present complex structure. This result supports the hypothesis that significant movement of the loop may depend on aglycone site being filled (Payan and Qian, J. Protein Chen. 22: 275, 2003). Structural analyses have shown that pancreatic alpha-amylases undergo an induced conformational change of the catalytic residue Asp300 upon substrate binding; in the present complex the catalytic residue is observed in its unliganded orientation. The results suggest that the induced reorientation is likely due to the presence of a sugar unit at subsite -1 and not linked to the closure of the flexible surface loop. The crystal structure was refined at 2.4 A resolution to an R factor of 17.55% (Rfree factor of 23.32%).
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The line below this paragraph, {{ABSTRACT_PUBMED_15517985}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15517985 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15517985}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of the pig pancreatic alpha-amylase complexed with rho-nitrophenyl-alpha-D-maltoside-flexibility in the active site., Zhuo H, Payan F, Qian M, Protein J. 2004 Aug;23(6):379-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15517985 15517985]
Crystal structure of the pig pancreatic alpha-amylase complexed with rho-nitrophenyl-alpha-D-maltoside-flexibility in the active site., Zhuo H, Payan F, Qian M, Protein J. 2004 Aug;23(6):379-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15517985 15517985]
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Structure of a pancreatic alpha-amylase bound to a substrate analogue at 2.03 A resolution., Qian M, Spinelli S, Driguez H, Payan F, Protein Sci. 1997 Nov;6(11):2285-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9385631 9385631]
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Crystal structure of the pig pancreatic alpha-amylase complexed with malto-oligosaccharides., Payan F, Qian M, J Protein Chem. 2003 Apr;22(3):275-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12962327 12962327]
[[Category: Alpha-amylase]]
[[Category: Alpha-amylase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Zhuo, H.]]
[[Category: Zhuo, H.]]
[[Category: Beta-alpha-barrel]]
[[Category: Beta-alpha-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:18:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:26:02 2008''

Revision as of 09:26, 29 July 2008

Image:1vah.png

Template:STRUCTURE 1vah

Crystal structure of the pig pancreatic-amylase complexed with r-nitrophenyl-a-D-maltoside

Template:ABSTRACT PUBMED 15517985

About this Structure

1VAH is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the pig pancreatic alpha-amylase complexed with rho-nitrophenyl-alpha-D-maltoside-flexibility in the active site., Zhuo H, Payan F, Qian M, Protein J. 2004 Aug;23(6):379-87. PMID:15517985

Structure of a pancreatic alpha-amylase bound to a substrate analogue at 2.03 A resolution., Qian M, Spinelli S, Driguez H, Payan F, Protein Sci. 1997 Nov;6(11):2285-96. PMID:9385631

Crystal structure of the pig pancreatic alpha-amylase complexed with malto-oligosaccharides., Payan F, Qian M, J Protein Chem. 2003 Apr;22(3):275-84. PMID:12962327

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