1p3r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1p3r.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1p3r.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1p3r| PDB=1p3r | SCENE= }}
{{STRUCTURE_1p3r| PDB=1p3r | SCENE= }}
-
'''Crystal structure of the phosphotyrosin binding domain(PTB) of mouse Disabled 1(Dab1)'''
+
===Crystal structure of the phosphotyrosin binding domain(PTB) of mouse Disabled 1(Dab1)===
-
==Overview==
+
<!--
-
Disabled (Dab) 1 and 2 are mammalian homologues of Drosophila DAB. Dab1 is a key cytoplasmic mediator in Reelin signaling that controls cell positioning in the developing central nervous system, whereas Dab2 is an adapter protein that plays a role in endocytosis. DAB family proteins possess an amino-terminal DAB homology (DH) domain that is similar to the phosphotyrosine binding/phosphotyrosine interaction (PTB/PI) domain. We have solved the structures of the DH domains of Dab2 (Dab2-DH) and Dab1 (Dab1-DH) in three different ligand forms, ligand-free Dab2-DH, the binary complex of Dab2-DH with the Asn-Pro-X-Tyr (NPXY) peptide of amyloid precursor protein (APP), and the ternary complex of Dab1-DH with the APP peptide and inositol 1,4,5-trisphosphate (Ins-1,4,5-P3, the head group of phosphatidylinositol-4,5-diphosphate (PtdIns-4,5-P2)). The similarity of these structures suggests that the rigid Dab DH domain maintains two independent pockets for binding of the APP/lipoprotein receptors and phosphoinositides. Mutagenesis confirmed the structural determinants specific for the NPXY sequence and PtdIns-4,5-P2 binding. NMR spectroscopy confirmed that the DH domain binds to Ins-1,4,5-P3 independent of the NPXY peptides. These findings suggest that simultaneous interaction of the rigid DH domain with the NPXY sequence and PtdIns-4,5-P2 plays a role in the attachment of Dab proteins to the APP/lipoprotein receptors and phosphoinositide-rich membranes.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12826668}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12826668 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12826668}}
==About this Structure==
==About this Structure==
Line 33: Line 37:
[[Category: Ptb]]
[[Category: Ptb]]
[[Category: Signaling protein]]
[[Category: Signaling protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:38:57 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:38:28 2008''

Revision as of 09:38, 29 July 2008

Image:1p3r.png

Template:STRUCTURE 1p3r

Crystal structure of the phosphotyrosin binding domain(PTB) of mouse Disabled 1(Dab1)

Template:ABSTRACT PUBMED 12826668

About this Structure

1P3R is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structures of the Dab homology domains of mouse disabled 1 and 2., Yun M, Keshvara L, Park CG, Zhang YM, Dickerson JB, Zheng J, Rock CO, Curran T, Park HW, J Biol Chem. 2003 Sep 19;278(38):36572-81. Epub 2003 Jun 24. PMID:12826668

Page seeded by OCA on Tue Jul 29 12:38:28 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p3r"
Personal tools