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1sbc

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{{STRUCTURE_1sbc| PDB=1sbc | SCENE= }}
{{STRUCTURE_1sbc| PDB=1sbc | SCENE= }}
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'''THE REFINED CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG AT 2.5 ANGSTROMS RESOLUTION'''
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===THE REFINED CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG AT 2.5 ANGSTROMS RESOLUTION===
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==Overview==
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We report here the X-ray crystal structure of native subtilisin Carlsberg, solved at 2.5 A resolution by molecular replacement and refined by restrained least squares to a crystallographic residual (Formula see text): of 0.206. we compare this structure to the crystal structure of subtilisin BPN'. We find that, despite 82 amino acid substitutions and one deletion in subtilisin Carlsberg relative to subtilisin BPN', the structures of these enzymes are remarkably similar. We calculate an r.m.s. difference between equivalent alpha-carbon positions in subtilisin Carlsberg and subtilisin BPN' of only 0.55 A. This confirms previous reports of extensive structural homology between these two subtilisins based on X-ray crystal structures of the complex of eglin-c with subtilisin Carlsberg [McPhalen, C.A., Schnebli, H.P. and James, M.N.G. (1985) FEBS Lett., 188, 55; Bode, W., Papamokos, E. and Musil, D. (1987) Eur. J. Biochem., 166, 673-692]. In addition, we find that the native active sites of subtilisins Carlsberg and BPN' are virtually identical. While conservative substitutions at residues 217 and 156 may have subtle effects on the environments of substrate-binding sites S1' and S1 respectively, we find no obvious structural correlate for reports that subtilisins Carlsberg and BPN' differ in their recognition of model substrates. In particular, we find no evidence that the hydrophobic binding pocket S1 in subtilisin Carlsberg is 'deeper', 'narrower' or 'less polar' than the corresponding binding site in subtilisin BPN'.
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{{ABSTRACT_PUBMED_3150541}}
==About this Structure==
==About this Structure==
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[[Category: Petsko, G A.]]
[[Category: Petsko, G A.]]
[[Category: Serine proteinase]]
[[Category: Serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:30:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:54:38 2008''

Revision as of 09:54, 29 July 2008

Image:1sbc.png

Template:STRUCTURE 1sbc

THE REFINED CRYSTAL STRUCTURE OF SUBTILISIN CARLSBERG AT 2.5 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 3150541

About this Structure

1SBC is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

The refined crystal structure of subtilisin Carlsberg at 2.5 A resolution., Neidhart DJ, Petsko GA, Protein Eng. 1988 Oct;2(4):271-6. PMID:3150541

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