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2ger

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[[Image:2ger.gif|left|200px]]
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{{STRUCTURE_2ger| PDB=2ger | SCENE= }}
{{STRUCTURE_2ger| PDB=2ger | SCENE= }}
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'''Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase'''
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===Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase===
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==Overview==
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Pyrroline-5-carboxylate reductase (P5CR) is a universal housekeeping enzyme that catalyzes the reduction of Delta(1)-pyrroline-5-carboxylate (P5C) to proline using NAD(P)H as the cofactor. The enzymatic cycle between P5C and proline is very important for the regulation of amino acid metabolism, intracellular redox potential, and apoptosis. Here, we present the 2.8 Angstroms resolution structure of the P5CR apo enzyme, its 3.1 Angstroms resolution ternary complex with NAD(P)H and substrate-analog. The refined structures demonstrate a decameric architecture with five homodimer subunits and ten catalytic sites arranged around a peripheral circular groove. Mutagenesis and kinetic studies reveal the pivotal roles of the dinucleotide-binding Rossmann motif and residue Glu221 in the human enzyme. Human P5CR is thermostable and the crystals were grown at 37 degrees C. The enzyme is implicated in oxidation of the anti-tumor drug thioproline.
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(as it appears on PubMed at http://www.pubmed.gov), where 16730026 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16730026}}
==About this Structure==
==About this Structure==
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[[Category: Rao, Z.]]
[[Category: Rao, Z.]]
[[Category: Crystal structure,oxidative mechanism of human pyrroline-5-carboxylate]]
[[Category: Crystal structure,oxidative mechanism of human pyrroline-5-carboxylate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:01:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:42:25 2008''

Revision as of 10:42, 29 July 2008

Image:2ger.png

Template:STRUCTURE 2ger

Crystal Structure and Oxidative Mechanism of Human Pyrroline-5-carboxylate Reductase

Template:ABSTRACT PUBMED 16730026

About this Structure

2GER is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human pyrroline-5-carboxylate reductase., Meng Z, Lou Z, Liu Z, Li M, Zhao X, Bartlam M, Rao Z, J Mol Biol. 2006 Jun 23;359(5):1364-77. Epub 2006 May 11. PMID:16730026

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