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2fik

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{{STRUCTURE_2fik| PDB=2fik | SCENE= }}
{{STRUCTURE_2fik| PDB=2fik | SCENE= }}
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'''Structure of a microbial glycosphingolipid bound to mouse CD1d'''
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===Structure of a microbial glycosphingolipid bound to mouse CD1d===
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==Overview==
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Natural killer T (NKT) cells provide an innate-type immune response upon T cell receptor interaction with CD1d-presented antigens. We demonstrate through equilibrium tetramer binding and antigen presentation assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galacturonosyl ceramide (GalA-GSL) is a NKT cell agonist that is significantly weaker than alpha-galactosylceramide (alpha-GalCer), the most potent known NKT agonist. For GalA-GSL, a shorter fatty acyl chain, an absence of the 4-OH on the sphingosine tail and a 6'-COOH group on the galactose moiety account for its observed antigenic potency. We further determined the crystal structure of mCD1d in complex with GalA-GSL at 1.8-A resolution. The overall binding mode of GalA-GSL to mCD1d is similar to that of the short-chain alpha-GalCer ligand PBS-25, but its sphinganine chain is more deeply inserted into the F' pocket due to alternate hydrogen-bonding interactions between the sphinganine 3-OH with Asp-80. Subsequently, a slight lateral shift (&gt;1 A) of the galacturonosyl head group is noted at the CD1 surface compared with the galactose of alpha-GalCer. Because the relatively short C(14) fatty acid of GalA-GSL does not fully occupy the A' pocket, a spacer lipid is found that stabilizes this pocket. The lipid spacer was identified by GC/MS as a mixture of saturated and monounsaturated palmitic acid (C(16)). Comparison of available crystal structures of alpha-anomeric glycosphingolipids now sheds light on the structural basis of their differential antigenic potency and has led to the design and synthesis of NKT cell agonists with enhanced cell-based stimulatory activities compared with alpha-GalCer.
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(as it appears on PubMed at http://www.pubmed.gov), where 16537470 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16537470}}
==About this Structure==
==About this Structure==
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[[Category: Nkt cell]]
[[Category: Nkt cell]]
[[Category: Tcr]]
[[Category: Tcr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:56:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:29:57 2008''

Revision as of 11:30, 29 July 2008

Image:2fik.png

Template:STRUCTURE 2fik

Structure of a microbial glycosphingolipid bound to mouse CD1d

Template:ABSTRACT PUBMED 16537470

About this Structure

2FIK is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Design of natural killer T cell activators: structure and function of a microbial glycosphingolipid bound to mouse CD1d., Wu D, Zajonc DM, Fujio M, Sullivan BA, Kinjo Y, Kronenberg M, Wilson IA, Wong CH, Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):3972-7. Epub 2006 Mar 6. PMID:16537470

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