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2dpe

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{{STRUCTURE_2dpe| PDB=2dpe | SCENE= }}
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'''Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution'''
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===Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution===
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==Overview==
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A 40kDa glycoprotein from dry secretion of sheep is implicated as a signaling factor and is named as SPS-40. This protein is secreted only during the early phase of involution when the drastic tissue remodeling occurs in the mammary gland. SPS-40 was purified from sheep dry secretions and crystallized using hanging drop vapour diffusion method. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with cell dimensions, a=62.7A, b=66.4A, c=107.5A. The protein was also cloned for the determination of its complete amino acid sequence. The three-dimensional structure of SPS-40 was determined by X-ray crystallographic method at 2.0A resolution. The structure revealed the presence of an N-linked glycan chain at Asn39. The protein adopts a conformation with a classical (beta/alpha)(8)-barrel fold of triosephosphate isomerase (TIM) (residues 1-237 and 310-360) with an insertion of a small (alpha+beta) domain (residues 240-307) similar to that observed in chitinases. However, the Leu substitution for Glu in the consensus catalytic sequence in SPS-40 causes a loss of chitinase activity. Furthermore, the sugar-binding groove in SPS-40 is distorted considerably from the standard chitin-binding site in chitinase enzymes and hence the binding of chitin-like oligosaccharides is considerably hampered. Three surface loops, His188-His197, Phe202-Arg212 and Phe244-Pro260 have exceptionally high values of B-factors (average=70.5A(2)), indicating the presence of a less defined region.
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{{ABSTRACT_PUBMED_16859926}}
==About this Structure==
==About this Structure==
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[[Category: Mammary gland secretion]]
[[Category: Mammary gland secretion]]
[[Category: Signaling protein]]
[[Category: Signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:07:14 2008''

Revision as of 12:07, 29 July 2008

Image:2dpe.png

Template:STRUCTURE 2dpe

Crystal structure of a secretory 40KDA glycoprotein from sheep at 2.0A resolution

Template:ABSTRACT PUBMED 16859926

About this Structure

2DPE is a Single protein structure of sequence from Ovis aries. This structure supersedes the now removed PDB entry 1r2v. Full crystallographic information is available from OCA.

Reference

Crystal structure of a secretory signalling glycoprotein from sheep at 2.0A resolution., Srivastava DB, Ethayathulla AS, Kumar J, Singh N, Sharma S, Das U, Srinivasan A, Singh TP, J Struct Biol. 2006 Dec;156(3):505-16. Epub 2006 Jun 8. PMID:16859926

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