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1xfu

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[[Image:1xfu.gif|left|200px]]
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{{STRUCTURE_1xfu| PDB=1xfu | SCENE= }}
{{STRUCTURE_1xfu| PDB=1xfu | SCENE= }}
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'''Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin'''
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===Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin===
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==Overview==
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Edema factor (EF), a key anthrax exotoxin, has an anthrax protective antigen-binding domain (PABD) and a calmodulin (CaM)-activated adenylyl cyclase domain. Here, we report the crystal structures of CaM-bound EF, revealing the architecture of EF PABD. CaM has N- and C-terminal domains and each domain can bind two calcium ions. Calcium binding induces the conformational change of CaM from closed to open. Structures of the EF-CaM complex show how EF locks the N-terminal domain of CaM into a closed conformation regardless of its calcium-loading state. This represents a mechanism of how CaM effector alters the calcium affinity of CaM and uncouples the conformational change of CaM from calcium loading. Furthermore, structures of EF-CaM complexed with nucleotides show that EF uses two-metal-ion catalysis, a prevalent mechanism in DNA and RNA polymerases. A histidine (H351) further facilitates the catalysis of EF by activating a water to deprotonate 3'OH of ATP. Mammalian adenylyl cyclases share no structural similarity with EF and they also use two-metal-ion catalysis, suggesting the catalytic mechanism-driven convergent evolution of two structurally diverse adenylyl cyclases.
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(as it appears on PubMed at http://www.pubmed.gov), where 15719022 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15719022}}
==About this Structure==
==About this Structure==
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[[Category: Zhukovskaya, N L.]]
[[Category: Zhukovskaya, N L.]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:58:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:23:22 2008''

Revision as of 12:23, 29 July 2008

Image:1xfu.png

Template:STRUCTURE 1xfu

Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin

Template:ABSTRACT PUBMED 15719022

About this Structure

1XFU is a Protein complex structure of sequences from Bacillus anthracis and Homo sapiens. Full crystallographic information is available from OCA.

Reference

Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor., Shen Y, Zhukovskaya NL, Guo Q, Florian J, Tang WJ, EMBO J. 2005 Mar 9;24(5):929-41. Epub 2005 Feb 17. PMID:15719022

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