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| - | [[Image:2pzo.jpg|left|200px]] | + | #REDIRECT [[3e2u]] This PDB entry is obsolete and replaced by 3e2u |
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| - | {{Structure
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| - | |PDB= 2pzo |SIZE=350|CAPTION= <scene name='initialview01'>2pzo</scene>, resolution 2.60Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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| - | |ACTIVITY=
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| - | |GENE= DCTN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CLIP1, CYLN1, RSN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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| - | |DOMAIN=
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| - | |RELATEDENTRY=[[2cp5|2CP5]], [[2hkn|2HKN]], [[2hkq|2HKQ]], [[1txq|1TXQ]], [[2hqh|2HQH]]
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pzo OCA], [http://www.ebi.ac.uk/pdbsum/2pzo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pzo RCSB]</span>
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| - | }}
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| - | '''Crystal structure of the zinc-knuckle 2 domain of human CLIP-170 in complex with CAP-Gly domain of human Dynactin-1 (p150-Glued)'''
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| - | ==Overview==
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| - | In all eukaryotes, CAP-Gly proteins control important cellular processes. The molecular mechanisms underlying the functions of CAP-Gly domains, however, are still poorly understood. Here we use the complex formed between the CAP-Gly domain of p150(glued) and the C-terminal zinc knuckle of CLIP170 as a model system to explore the structure-function relationship of CAP-Gly-mediated protein interactions. We demonstrate that the conserved GKNDG motif of CAP-Gly domains is responsible for targeting to the C-terminal EEY/F sequence motifs of CLIP170, EB proteins and microtubules. The CAP-Gly-EEY/F interaction is essential for the recruitment of the dynactin complex by CLIP170 and for activation of CLIP170. Our findings define the molecular basis of CAP-Gly domain function, including the tubulin detyrosination-tyrosination cycle. They further establish fundamental roles for the interaction between CAP-Gly proteins and C-terminal EEY/F sequence motifs in regulating complex and dynamic cellular processes.
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| - | ==Disease==
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| - | Known disease associated with this structure: Neuropathy, distal hereditary motor, type VIIB OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601143 601143]], Amyotrophic lateral sclerosis, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601143 601143]]
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| - | ==About this Structure==
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| - | 2PZO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PZO OCA].
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| - | ==Reference==
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| - | Structure-function relationship of CAP-Gly domains., Weisbrich A, Honnappa S, Jaussi R, Okhrimenko O, Frey D, Jelesarov I, Akhmanova A, Steinmetz MO, Nat Struct Mol Biol. 2007 Oct;14(10):959-67. Epub 2007 Sep 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17828277 17828277]
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| - | [[Category: Homo sapiens]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Honnappa, S.]]
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| - | [[Category: Steinmetz, M O.]]
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| - | [[Category: Weisbrich, A.]]
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| - | [[Category: +tip protein complex structure]]
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| - | [[Category: autoinhibition]]
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| - | [[Category: clip-170/restin]]
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| - | [[Category: cytoskeleton associated protein]]
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| - | [[Category: dynactin]]
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| - | [[Category: p150glued]]
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| - | [[Category: protein binding]]
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| - | [[Category: structural protein microtubule binding]]
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| - | [[Category: zinc-knuckle]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:42:46 2008''
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