2bdz
From Proteopedia
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{{STRUCTURE_2bdz| PDB=2bdz | SCENE= }} | {{STRUCTURE_2bdz| PDB=2bdz | SCENE= }} | ||
| - | + | ===Mexicain from Jacaratia mexicana=== | |
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| - | ==Overview== | ||
| - | Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2BDZ is a | + | 2BDZ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:15502326</ref><references group="xtra"/> | |
[[Category: Jacaratia mexicana]] | [[Category: Jacaratia mexicana]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Garcia-Ruiz, J M.]] | [[Category: Garcia-Ruiz, J M.]] | ||
[[Category: Gavira, J A.]] | [[Category: Gavira, J A.]] | ||
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[[Category: Papain-like]] | [[Category: Papain-like]] | ||
[[Category: Peptidase_c1]] | [[Category: Peptidase_c1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:48:22 2009'' | ||
Revision as of 16:48, 16 February 2009
Mexicain from Jacaratia mexicana
About this Structure
2BDZ is a 4 chains structure of sequences from Jacaratia mexicana. Full crystallographic information is available from OCA.
Reference
- Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM. Purification, crystallization and preliminary X-ray analysis of mexicain. Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326 doi:10.1107/S0907444904021638
Page seeded by OCA on Mon Feb 16 18:48:22 2009
