This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2bdz
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[Image:2bdz. | + | {{Seed}} |
| + | [[Image:2bdz.png|left|200px]] | ||
<!-- | <!-- | ||
| Line 9: | Line 10: | ||
{{STRUCTURE_2bdz| PDB=2bdz | SCENE= }} | {{STRUCTURE_2bdz| PDB=2bdz | SCENE= }} | ||
| - | + | ===Mexicain from Jacaratia mexicana=== | |
| - | |||
| - | ==Overview== | ||
| - | Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2BDZ is a | + | 2BDZ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:15502326</ref><references group="xtra"/> | |
[[Category: Jacaratia mexicana]] | [[Category: Jacaratia mexicana]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Garcia-Ruiz, J M.]] | [[Category: Garcia-Ruiz, J M.]] | ||
[[Category: Gavira, J A.]] | [[Category: Gavira, J A.]] | ||
| Line 31: | Line 28: | ||
[[Category: Papain-like]] | [[Category: Papain-like]] | ||
[[Category: Peptidase_c1]] | [[Category: Peptidase_c1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:48:22 2009'' | ||
Revision as of 16:48, 16 February 2009
Mexicain from Jacaratia mexicana
About this Structure
2BDZ is a 4 chains structure of sequences from Jacaratia mexicana. Full crystallographic information is available from OCA.
Reference
- Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM. Purification, crystallization and preliminary X-ray analysis of mexicain. Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326 doi:10.1107/S0907444904021638
Page seeded by OCA on Mon Feb 16 18:48:22 2009
