2ncd
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 2NCD is a | + | 2NCD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. The April 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Kinesin'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_4 10.2210/rcsb_pdb/mom_2005_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NCD OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:9796817</ref><references group="xtra"/> | |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Kinesin]] | [[Category: Kinesin]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Case, R B.]] | [[Category: Case, R B.]] | ||
[[Category: Dai, S C.]] | [[Category: Dai, S C.]] | ||
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[[Category: Ncd]] | [[Category: Ncd]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:03:12 2009'' |
Revision as of 08:03, 17 February 2009
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| 2ncd, resolution 2.50Å () | |||||||||
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| Ligands: | , | ||||||||
| Gene: | NCD (Drosophila melanogaster) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER
Motor proteins of the kinesin superfamily transport intracellular cargo along microtubules. Although different kinesin proteins share 30-50% amino-acid identity in their motor catalytic cores, some move to the plus end of microtubules whereas others travel in the opposite direction. Crystal structures of the catalytic cores of conventional kinesin (a plus-end-directed motor involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregation) are nearly identical; therefore, the structural basis for their opposite directions of movement is unknown. Here we show that the ncd 'neck' made up of 13 class-specific residues next to the superfamily-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these neck residues reverses the direction of ncd motion. By solving the 2.5 A structure of a functional ncd dimer, we show that the ncd neck (a coiled-coil) differs from the corresponding region in the kinesin neck (an interrupted beta-strand), although both necks interact with similar elements in the catalytic cores. The distinct neck architectures also confer different symmetries to the ncd and kinesin dimers and position these motors with appropriate directional bias on the microtubule.
Direction determination in the minus-end-directed kinesin motor ncd., Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ, Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2NCD is a 1 chain structure of sequence from Drosophila melanogaster. The April 2005 RCSB PDB Molecule of the Month feature on Kinesin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_4. Full crystallographic information is available from OCA.
Reference
- Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ. Direction determination in the minus-end-directed kinesin motor ncd. Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817 doi:10.1038/27463
Page seeded by OCA on Tue Feb 17 10:03:12 2009
