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Proposed article title: Release of RING from Cullin by NEDD8ylation
Proposed article title: Release of RING from Cullin by NEDD8ylation
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<applet load='Image:3dpl_3dqv_acr_morph.pdb' size='500' frame='true' align='right' caption='Insert caption here' />
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<applet load='3dpl_3dqv_acr_morph.pdb' size='500' frame='true' align='right' caption='Insert caption here' />
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At right is a morph<ref>[[Image:3dpl_3dqv_acr_morph.pdb]]</ref><ref>Methods</ref> from a Cullin-RING ligase complex ([[3dpl]]) to the same complex after NEDD8ylation (covalent addition of the NEDD8 ubiquitin-like chain to LysXXX of Cullin; [[3dqv]]). NEDD8 binds and rotates the C-terminal domain of Cullin, releasing the folded RING domain from its Cullin-binding site. RING remains attached to Cullin by a coil at the RING X-terminus which is embedded in Cullin.
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At right is a morph
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<ref>[[Image:3dpl_3dqv_acr_morph.pdb]]</ref>
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<ref>Methods</ref>
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from a Cullin-RING ligase complex ([[3dpl]]) to the same complex after NEDD8ylation (covalent addition of the NEDD8 ubiquitin-like chain to LysXXX of Cullin; [[3dqv]]). NEDD8 binds and rotates the C-terminal domain of Cullin, releasing the folded RING domain from its Cullin-binding site. RING remains attached to Cullin by a coil at the RING X-terminus which is embedded in Cullin.
==Notes and References==
==Notes and References==
<references />
<references />

Revision as of 03:17, 22 February 2009

This article is under collaborative development by Eric Martz (UMass) and Raymond J. Deshaies and his team (CalTech). Please do not edit this page unless you are a member of the development team.. Once completed, this page may be moved to a permanent article title.

Proposed article title: Release of RING from Cullin by NEDD8ylation

Insert caption here

Drag the structure with the mouse to rotate

At right is a morph [1] [2] from a Cullin-RING ligase complex (3dpl) to the same complex after NEDD8ylation (covalent addition of the NEDD8 ubiquitin-like chain to LysXXX of Cullin; 3dqv). NEDD8 binds and rotates the C-terminal domain of Cullin, releasing the folded RING domain from its Cullin-binding site. RING remains attached to Cullin by a coil at the RING X-terminus which is embedded in Cullin.

Notes and References

  1. Image:3dpl 3dqv acr morph.pdb
  2. Methods
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