1vys
From Proteopedia
(New page: 200px<br /> <applet load="1vys" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vys, resolution 1.8Å" /> '''STUCTURE OF PENTAERY...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1VYS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]] with FMN and TNF as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VYS OCA]]. | + | 1VYS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]] with FMN and TNF as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VYS OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: steroid binding]] | [[Category: steroid binding]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:22:48 2007'' |
Revision as of 06:18, 30 October 2007
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STUCTURE OF PENTAERYTHRITOL TETRANIRATE REDUCTASE W102Y MUTANT AND COMPLEXED WITH PICRIC ACID
Overview
The structure of pentaerythritol tetranitrate (PETN) reductase in complex, with the nitroaromatic substrate picric acid determined previously at 1.55, A resolution indicated additional electron density between the indole ring, of residue Trp-102 and the nitro group at C-6 of picrate. The data, suggested the presence of an unusual bond between substrate and the, tryptophan side chain. Herein, we have extended the resolution of the PETN, reductase-picric acid complex to 0.9 A. This high-resolution analysis, indicates that the active site is partially occupied with picric acid and, that the anomalous density seen in the original study is attributed to the, population of multiple conformational states of Trp-102 and not a formal, covalent bond between the indole ring of Trp-102 and picric ... [(full description)]
About this Structure
1VYS is a [Single protein] structure of sequence from [Enterobacter cloacae] with FMN and TNF as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degradation., Khan H, Barna T, Harris RJ, Bruce NC, Barsukov I, Munro AW, Moody PC, Scrutton NS, J Biol Chem. 2004 Jul 16;279(29):30563-72. Epub 2004 May 5. PMID:15128738
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