Sandbox 9

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== Headline text ==
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[[Image:1ab4.png|left|200px]]
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[[Image:1ema 9.gif|350px]]
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Green fluorescent protein ('''GFP'''), originally isolated from the jellyfish Aequorea victoria (PDB entry [[1ema]]), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.
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== Exploring the Structure ==
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The line below this paragraph, containing "STRUCTURE_1ab4", creates the "Structure Box" on the page.
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<applet load='1ema' size='300' frame='true' align='right' caption='Insert caption here scene='Sandbox_9/Gfp/2'>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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-->
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{{STRUCTURE_1ab4| PDB=1ab4 | SCENE= }}
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===59KDA FRAGMENT OF GYRASE A FROM E. COLI===
 
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'''GFP''' is a beta barrel protein with 11 beta sheets. It is a 26.9kDa protein made up of 238 amino acids. The chromophore, responsible for the fluorescent properties of the protein, is buried inside the beta barrel as part of the central alpha helix passing through the barrel. The chromophore forms via spontaneous cyclization and oxidation of three residues in the central alpha helix: -Thr65 (or Ser65)-Tyr66-Gly67. This cyclization and oxidation creates the chromophore's five-membered ring via a new bond between the threonine and the glycine residues.
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The line below this paragraph, {{ABSTRACT_PUBMED_9278055}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9278055 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9278055}}
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==About this Structure==
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=== Biological Significance ===
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1AB4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AB4 OCA].
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'''GFP''' <scene name='Sandbox_9/Gfp/2'> GFP Chromophore </scene> is a useful marker protein that can be used for keeping track of gene expression in a tissue specific manner.<ref>PMID: 8703075 </ref>
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==Reference==
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==References==
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<ref group="xtra">PMID:9278055</ref><references group="xtra"/>
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<references/>

Current revision

Headline text

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria (PDB entry 1ema), fluorsceses green (509nm) when exposed to blue light (395nm and 475nm). It is one of the most important proteins used in biological research because it can be used to tag otherwise invisible gene products of interest and thus observe their existence, location and movement.

Exploring the Structure

caption

Drag the structure with the mouse to rotate
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