1oxs

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(New page: 200px<br /><applet load="1oxs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oxs, resolution 1.65&Aring;" /> '''Crystal structure of...)
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[[Image:1oxs.jpg|left|200px]]<br /><applet load="1oxs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oxs, resolution 1.65&Aring;" />
 
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'''Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus'''<br />
 
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==Overview==
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==Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus==
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The ABC-ATPase GlcV energizes a binding protein-dependent ABC transporter, that mediates glucose uptake in Sulfolobus solfataricus. Here, we report, high-resolution crystal structures of GlcV in different states along its, catalytic cycle: distinct monomeric nucleotide-free states and monomeric, complexes with ADP-Mg(2+) as a product-bound state, and with AMPPNP-Mg(2+), as an ATP-like bound state. The structure of GlcV consists of a typical, ABC-ATPase domain, comprising two subdomains, connected by a linker region, to a C-terminal domain of unknown function. Comparisons of the, nucleotide-free and nucleotide-bound structures of GlcV reveal, re-orientations of the ABCalpha subdomain and the C-terminal domain, relative to the ABCalpha/beta subdomain, and switch-like rearrangements in, the P-loop and Q-loop regions. Additionally, large conformational, differences are observed between the GlcV structures and those of other, ABC-ATPases, further emphasizing the inherent flexibility of these, proteins. Notably, a comparison of the monomeric AMPPNP-Mg(2+)-bound GlcV, structure with that of the dimeric ATP-Na(+)-bound LolD-E171Q mutant, reveals a +/-20 degrees rigid body re-orientation of the ABCalpha, subdomain relative to the ABCalpha/beta subdomain, accompanied by a local, conformational difference in the Q-loop. We propose that these differences, represent conformational changes that may have a role in the mechanism of, energy-transduction and/or allosteric control of the ABC-ATPase activity, in bacterial importers.
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<StructureSection load='1oxs' size='340' side='right'caption='[[1oxs]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1oxs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OXS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oxs OCA], [https://pdbe.org/1oxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oxs RCSB], [https://www.ebi.ac.uk/pdbsum/1oxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oxs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLCV_SACS2 GLCV_SACS2] Part of the ABC transporter complex GlcSTUV involved in glucose uptake (Probable). Responsible for energy coupling to the transport system (PubMed:12823973, PubMed:14607117). In vitro, as a free subunit, exhibits a constitutive ATPase activity (PubMed:11807278).<ref>PMID:11807278</ref> <ref>PMID:12823973</ref> <ref>PMID:14607117</ref> <ref>PMID:10400586</ref> <ref>PMID:11260467</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ox/1oxs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oxs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OXS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with IOD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OXS OCA].
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structures of the ATPase subunit of the glucose ABC transporter from Sulfolobus solfataricus: nucleotide-free and nucleotide-bound conformations., Verdon G, Albers SV, Dijkstra BW, Driessen AJ, Thunnissen AM, J Mol Biol. 2003 Jul 4;330(2):343-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12823973 12823973]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Sulfolobus solfataricus]]
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[[Category: Large Structures]]
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[[Category: Albers, S.V.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Albers SV]]
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[[Category: Driessen, A.J.]]
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[[Category: Dijkstra BW]]
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[[Category: Thunnissen, A.M.]]
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[[Category: Driessen AJ]]
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[[Category: Verdon, G.]]
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[[Category: Thunnissen AM]]
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[[Category: IOD]]
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[[Category: Verdon G]]
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[[Category: abc-atpase]]
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[[Category: atp-binding cassette]]
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[[Category: atpase]]
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[[Category: glcv]]
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[[Category: sulfolobus solfataricus]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:38:20 2007''
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Current revision

Crystal structure of GlcV, the ABC-ATPase of the glucose ABC transporter from Sulfolobus solfataricus

PDB ID 1oxs

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