2wm9

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{{Seed}}
 
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[[Image:2wm9.jpg|left|200px]]
 
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==Structure of the complex between DOCK9 and Cdc42.==
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The line below this paragraph, containing "STRUCTURE_2wm9", creates the "Structure Box" on the page.
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<StructureSection load='2wm9' size='340' side='right'caption='[[2wm9]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wm9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WM9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2wm9| PDB=2wm9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wm9 OCA], [https://pdbe.org/2wm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wm9 RCSB], [https://www.ebi.ac.uk/pdbsum/2wm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wm9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DOCK9_HUMAN DOCK9_HUMAN] Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.<ref>PMID:12172552</ref> <ref>PMID:19745154</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wm/2wm9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wm9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Activation of Rho guanosine triphosphatases (GTPases) to the guanine triphosphate (GTP)-bound state is a critical event in their regulation of the cytoskeleton and cell signaling. Members of the DOCK family of guanine nucleotide exchange factors (GEFs) are important activators of Rho GTPases, but the mechanism of activation by their catalytic DHR2 domain is unknown. Through structural analysis of DOCK9-Cdc42 complexes, we identify a nucleotide sensor within the alpha10 helix of the DHR2 domain that contributes to release of guanine diphosphate (GDP) and then to discharge of the activated GTP-bound Cdc42. Magnesium exclusion, a critical factor in promoting GDP release, is mediated by a conserved valine residue within this sensor, whereas binding of GTP-Mg2+ to the nucleotide-free complex results in magnesium-inducing displacement of the sensor to stimulate discharge of Cdc42-GTP. These studies identify an unusual mechanism of GDP release and define the complete GEF catalytic cycle from GDP dissociation followed by GTP binding and discharge of the activated GTPase.
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===STRUCTURE OF THE COMPLEX BETWEEN DOCK9 AND CDC42.===
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Activation of Rho GTPases by DOCK exchange factors is mediated by a nucleotide sensor.,Yang J, Zhang Z, Roe SM, Marshall CJ, Barford D Science. 2009 Sep 11;325(5946):1398-402. PMID:19745154<ref>PMID:19745154</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wm9" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19745154}}, adds the Publication Abstract to the page
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*[[Dedicator of cytokinesis protein 3D structures|Dedicator of cytokinesis protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19745154 is the PubMed ID number.
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_19745154}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2WM9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WM9 OCA].
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==Reference==
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<ref group="xtra">PMID:19745154</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Barford, D.]]
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[[Category: Large Structures]]
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[[Category: Roe, S M.]]
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[[Category: Barford D]]
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[[Category: Yang, J.]]
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[[Category: Roe SM]]
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[[Category: Alternative splicing]]
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[[Category: Yang J]]
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[[Category: Cdc42]]
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[[Category: Cell cycle]]
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[[Category: Cell membrane]]
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[[Category: Coiled coil]]
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[[Category: Dock9]]
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[[Category: Gef]]
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[[Category: Gtp-binding]]
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[[Category: Guanine-nucleotide releasing factor]]
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[[Category: Lipoprotein]]
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[[Category: Methylation]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Prenylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 08:51:26 2009''
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Current revision

Structure of the complex between DOCK9 and Cdc42.

PDB ID 2wm9

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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