1x8w

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(New page: 200px<br /><applet load="1x8w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x8w, resolution 3.8&Aring;" /> '''Structure of the Tetr...)
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[[Image:1x8w.gif|left|200px]]<br /><applet load="1x8w" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1x8w, resolution 3.8&Aring;" />
 
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'''Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site'''<br />
 
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==Overview==
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==Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site==
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The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its, self-splicing reaction, this ribozyme catalyzes phosphoryl transfer, between guanosine and a substrate RNA strand. Here we report the refined, crystal structure of an active Tetrahymena ribozyme in the absence of its, RNA substrate at 3.8 A resolution. The 3'-terminal guanosine (omegaG), which serves as the attacking group for RNA cleavage, forms a coplanar, base triple with the G264-C311 base pair, and this base triple is, sandwiched by three other base triples. In addition, a metal ion is, present in the active site, contacting or positioned close to the ribose, of the omegaG and five phosphates. All of these phosphates have been shown, to be important for catalysis. Therefore, we provide a picture of how the, ribozyme active site positions both a catalytic metal ion and the, nucleophilic guanosine for catalysis prior to binding its RNA substrate.
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<StructureSection load='1x8w' size='340' side='right'caption='[[1x8w]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1x8w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X8W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8w OCA], [https://pdbe.org/1x8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x8w RCSB], [https://www.ebi.ac.uk/pdbsum/1x8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8w ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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1X8W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1X8W OCA].
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15525509 15525509]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Tetrahymena thermophila]]
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[[Category: Cech, T.R.]]
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[[Category: Cech TR]]
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[[Category: Gooding, A.R.]]
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[[Category: Gooding AR]]
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[[Category: Guo, F.]]
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[[Category: Guo F]]
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[[Category: MG]]
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[[Category: active site]]
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[[Category: base triples]]
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[[Category: catalytic mechanism]]
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[[Category: catalytic rna]]
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[[Category: conformational changes]]
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[[Category: crystal structure]]
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[[Category: group i intron]]
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[[Category: guanosine binding site]]
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[[Category: metal ions]]
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[[Category: ribozyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:39:22 2007''
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Current revision

Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site

PDB ID 1x8w

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