1koc

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(New page: 200px<br /><applet load="1koc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1koc" /> '''RNA APTAMER COMPLEXED WITH ARGININE, NMR'''<...)
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[[Image:1koc.jpg|left|200px]]<br /><applet load="1koc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1koc" />
 
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'''RNA APTAMER COMPLEXED WITH ARGININE, NMR'''<br />
 
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==Overview==
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==RNA APTAMER COMPLEXED WITH ARGININE, NMR==
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In a previous study, an RNA aptamer for the specific recognition of, arginine was evolved from a parent sequence that bound citrulline, specifically. The two RNAs differ at only 3 positions out of 44. The, solution structures of the two aptamers complexed to their cognate amino, acids have now been determined by two-dimensional nuclear magnetic, resonance spectroscopy. Both aptamers contain two asymmetrical internal, loops that are not well ordered in the free RNA but that fold into a, compact structure upon ligand binding. Those nucleotides common to both, RNAs include a conserved cluster of purine residues, three of which form, an uneven plane containing a G:G pair, and two other residues nearly, perpendicular to that surface. Two of the three variant nucleotides are, stacked on the cluster of purines and form a triple contact to the amino, acid side chain, whereas the edge of the third variant nucleotide is, capping the binding pocket.
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<StructureSection load='1koc' size='340' side='right'caption='[[1koc]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1koc]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KOC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1koc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1koc OCA], [https://pdbe.org/1koc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1koc RCSB], [https://www.ebi.ac.uk/pdbsum/1koc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1koc ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In a previous study, an RNA aptamer for the specific recognition of arginine was evolved from a parent sequence that bound citrulline specifically. The two RNAs differ at only 3 positions out of 44. The solution structures of the two aptamers complexed to their cognate amino acids have now been determined by two-dimensional nuclear magnetic resonance spectroscopy. Both aptamers contain two asymmetrical internal loops that are not well ordered in the free RNA but that fold into a compact structure upon ligand binding. Those nucleotides common to both RNAs include a conserved cluster of purine residues, three of which form an uneven plane containing a G:G pair, and two other residues nearly perpendicular to that surface. Two of the three variant nucleotides are stacked on the cluster of purines and form a triple contact to the amino acid side chain, whereas the edge of the third variant nucleotide is capping the binding pocket.
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==About this Structure==
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Structural basis of ligand discrimination by two related RNA aptamers resolved by NMR spectroscopy.,Yang Y, Kochoyan M, Burgstaller P, Westhof E, Famulok M Science. 1996 May 31;272(5266):1343-7. PMID:8650546<ref>PMID:8650546</ref>
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1KOC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ARG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KOC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis of ligand discrimination by two related RNA aptamers resolved by NMR spectroscopy., Yang Y, Kochoyan M, Burgstaller P, Westhof E, Famulok M, Science. 1996 May 31;272(5266):1343-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8650546 8650546]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1koc" style="background-color:#fffaf0;"></div>
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[[Category: Burgstaller, P.]]
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== References ==
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[[Category: Famulok, M.]]
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<references/>
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[[Category: Kochoyan, M.]]
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__TOC__
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[[Category: Westhof, E.]]
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</StructureSection>
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[[Category: Yang, Y.S.]]
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[[Category: Large Structures]]
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[[Category: ARG]]
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[[Category: Burgstaller P]]
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[[Category: complex (rna aptamer/peptide)]]
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[[Category: Famulok M]]
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[[Category: in vitro selected rna]]
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[[Category: Kochoyan M]]
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[[Category: rna aptamer]]
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[[Category: Westhof E]]
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[[Category: Yang YS]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:39:57 2007''
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RNA APTAMER COMPLEXED WITH ARGININE, NMR

PDB ID 1koc

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