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1ozp

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(New page: 200px<br /><applet load="1ozp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ozp, resolution 1.70&Aring;" /> '''Crystal Structure of...)
Current revision (08:04, 14 February 2024) (edit) (undo)
 
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[[Image:1ozp.jpg|left|200px]]<br /><applet load="1ozp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ozp, resolution 1.70&Aring;" />
 
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'''Crystal Structure of Rv0819 from Mycobacterium tuberculosis MshD-Mycothiol Synthase Acetyl-Coenzyme A Complex.'''<br />
 
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==Overview==
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==Crystal Structure of Rv0819 from Mycobacterium tuberculosis MshD-Mycothiol Synthase Acetyl-Coenzyme A Complex.==
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Mycothiol is the predominant low-molecular weight thiol produced by, actinomycetes, including Mycobacterium tuberculosis. The last reaction in, the biosynthetic pathway for mycothiol is catalyzed by mycothiol synthase, (MshD), which acetylates the cysteinyl amine of, cysteine-glucosamine-inositol (Cys-GlcN-Ins). The crystal structure of, MshD was determined in the presence of coenzyme A and acetyl-CoA. MshD, consists of two tandem-repeated domains, each exhibiting the Gcn5-related, N-acetyltransferase (GNAT) fold. These two domains superimpose with a, root-mean-square deviation of 1.7 A over 88 residues, and each was found, to bind one molecule of coenzyme, although the binding sites are quite, different. The C-terminal domain has a similar active site to many GNAT, members in which the acetyl group of the coenzyme is presented to an open, active site slot. However, acetyl-CoA bound to the N-terminal domain is, buried, and is apparently not positioned to promote acetyl transfer. A, modeled substrate complex indicates that Cys-GlcN-Ins would only fill a, portion of a negatively charged channel located between the two domains., This is the first structure determined for an enzyme involved in the, biosynthesis of mycothiol.
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<StructureSection load='1ozp' size='340' side='right'caption='[[1ozp]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ozp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZP FirstGlance]. <br>
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1OZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with ACO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OZP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozp OCA], [https://pdbe.org/1ozp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozp RCSB], [https://www.ebi.ac.uk/pdbsum/1ozp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozp ProSAT]</span></td></tr>
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Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases., Vetting MW, Roderick SL, Yu M, Blanchard JS, Protein Sci. 2003 Sep;12(9):1954-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12930994 12930994]
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</table>
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[[Category: Mycobacterium tuberculosis h37rv]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/MSHD_MYCTU MSHD_MYCTU] Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol.<ref>PMID:12375100</ref>
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[[Category: Blanchard, J.S.]]
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== Evolutionary Conservation ==
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[[Category: Roderick, S.L.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Vetting, M.W.]]
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Check<jmol>
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[[Category: Yu, M.]]
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<jmolCheckbox>
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[[Category: ACO]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozp_consurf.spt"</scriptWhenChecked>
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[[Category: acetyl coenzyme a complex]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: acetyltransferase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: gnat fold]]
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</jmolCheckbox>
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[[Category: mshd]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ozp ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:44:03 2007''
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Blanchard JS]]
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[[Category: Roderick SL]]
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[[Category: Vetting MW]]
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[[Category: Yu M]]

Current revision

Crystal Structure of Rv0819 from Mycobacterium tuberculosis MshD-Mycothiol Synthase Acetyl-Coenzyme A Complex.

PDB ID 1ozp

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