3hda

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{{Seed}}
 
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[[Image:3hda.png|left|200px]]
 
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==PrtC methionine mutants: M226A_DESY==
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The line below this paragraph, containing "STRUCTURE_3hda", creates the "Structure Box" on the page.
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<StructureSection load='3hda' size='340' side='right'caption='[[3hda]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hda]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HDA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.131&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_3hda| PDB=3hda | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hda OCA], [https://pdbe.org/3hda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hda RCSB], [https://www.ebi.ac.uk/pdbsum/3hda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hda ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRTC_DICCH PRTC_DICCH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hd/3hda_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hda ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Abstract The metzincins constitute a subclan of metalloproteases possessing a HEXXHXXGXXH/D zinc-binding consensus sequence where the three histidines are zinc ligands and the glutamic acid the catalytic base. A completely conserved methionine is located downstream of this motif. Families of the metzincin clan comprise, besides others, astacins, ADAMS proteases, matrix metalloproteases and serralysins. The latter are extracellular 50 kDa proteases secreted by Gram-negative bacteria via a type I secretion system. While there is a large body of structural and biochemical information available, the function of the conserved methionine has not been convincingly clarified yet. Here we present the crystal structures of a number of mutants of the serralysin member protease C with the conserved methionine being replaced by Ile, Ala and His. Together with our former report on the leucine and cysteine mutants, we demonstrate here that replacement of the methionine side chain results in an increasing distortion of the zinc-binding geometry, especially pronounced in the chi(2) angles of the first and third histidine of the consensus sequence. This is correlated with an increasing loss of proteolytic activity and a sharp increase of flexibility of large segments of the polypeptide chain.
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===PrtC methionine mutants: M226A_DESY===
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Metzincin's canonical methionine is responsible for the structural integrity of the zinc-binding site.,Oberholzer AE, Bumann M, Hege T, Russo S, Baumann U Biol Chem. 2009 Jun 27. PMID:19558324<ref>PMID:19558324</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19558324}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3hda" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19558324 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19558324}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Dickeya chrysanthemi]]
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3HDA is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HDA OCA].
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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==Reference==
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[[Category: Baumann U]]
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<ref group="xtra">PMID:19558324</ref><references group="xtra"/>
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[[Category: Bumann M]]
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[[Category: Erwinia chrysanthemi]]
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[[Category: Hege T]]
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[[Category: Baumann, U.]]
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[[Category: Oberholzer AE]]
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[[Category: Bumann, M.]]
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[[Category: Russo S]]
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[[Category: Hege, T.]]
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[[Category: Oberholzer, A E.]]
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[[Category: Russo, S.]]
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[[Category: Beta roll]]
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[[Category: Calcium]]
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[[Category: Hydrolase]]
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[[Category: Met-turn]]
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[[Category: Metal-binding]]
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[[Category: Metalloprotease]]
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[[Category: Metzincin]]
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[[Category: Protease]]
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[[Category: Secreted]]
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[[Category: Zinc]]
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[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 30 08:41:37 2009''
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Current revision

PrtC methionine mutants: M226A_DESY

PDB ID 3hda

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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