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1p0u

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(New page: 200px<br /><applet load="1p0u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p0u" /> '''Sheared G/C Base Pair'''<br /> ==Overview==...)
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[[Image:1p0u.gif|left|200px]]<br /><applet load="1p0u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p0u" />
 
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'''Sheared G/C Base Pair'''<br />
 
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==Overview==
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==Sheared G/C Base Pair==
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Stable DNA loop structures closed by a novel G.C base-pair have been, determined for the single-residue d(GXC) loops (X=A, T, G or C) in, low-salt solution by high-resolution nuclear magnetic resonance (NMR), techniques. The closing G.C base-pair in these loops is not of the, canonical Watson-Crick type, but adopts instead a unique sheared-type, (trans Watson-Crick/sugar-edge) pairing, like those occurring in the, sheared mismatched G.A or A.C base-pair, to draw the two opposite strands, together. The cytidine residue in the closing base-pair is transformed, into the rare syn domain to form two H-bonds with the guanine base and to, prevent the steric clash between the G 2NH(2) and the C H-5 protons., Besides, the sugar pucker of the syn cytidine is still located in the, regular C2'-endo domain, unlike the C3'-endo domain adopted for the, pyrimidines of the out-of-alternation left-handed Z-DNA structure. The, facile formation of the compact d(GXC) loops closed by a unique, sheared-type G(anti).C(syn) base-pair demonstrates the great potential of, the single-stranded d(GXC) triplet repeats to fold into stable hairpins.
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<StructureSection load='1p0u' size='340' side='right'caption='[[1p0u]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P0U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p0u OCA], [https://pdbe.org/1p0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p0u RCSB], [https://www.ebi.ac.uk/pdbsum/1p0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p0u ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Stable DNA loop structures closed by a novel G.C base-pair have been determined for the single-residue d(GXC) loops (X=A, T, G or C) in low-salt solution by high-resolution nuclear magnetic resonance (NMR) techniques. The closing G.C base-pair in these loops is not of the canonical Watson-Crick type, but adopts instead a unique sheared-type (trans Watson-Crick/sugar-edge) pairing, like those occurring in the sheared mismatched G.A or A.C base-pair, to draw the two opposite strands together. The cytidine residue in the closing base-pair is transformed into the rare syn domain to form two H-bonds with the guanine base and to prevent the steric clash between the G 2NH(2) and the C H-5 protons. Besides, the sugar pucker of the syn cytidine is still located in the regular C2'-endo domain, unlike the C3'-endo domain adopted for the pyrimidines of the out-of-alternation left-handed Z-DNA structure. The facile formation of the compact d(GXC) loops closed by a unique sheared-type G(anti).C(syn) base-pair demonstrates the great potential of the single-stranded d(GXC) triplet repeats to fold into stable hairpins.
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==About this Structure==
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Sheared-type G(anti).C(syn) base-pair: a unique d(GXC) loop closure motif.,Chin KH, Chou SH J Mol Biol. 2003 May 30;329(2):351-61. PMID:12758081<ref>PMID:12758081</ref>
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1P0U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P0U OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Sheared-type G(anti).C(syn) base-pair: a unique d(GXC) loop closure motif., Chin KH, Chou SH, J Mol Biol. 2003 May 30;329(2):351-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12758081 12758081]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1p0u" style="background-color:#fffaf0;"></div>
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[[Category: Chin, K.H.]]
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== References ==
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[[Category: Chou, S.H.]]
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<references/>
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[[Category: nmr]]
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__TOC__
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[[Category: sheared gc base pair]]
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</StructureSection>
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[[Category: single-residue loop]]
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[[Category: Large Structures]]
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[[Category: syn cytidine]]
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[[Category: Chin KH]]
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[[Category: Chou SH]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:46:47 2007''
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Sheared G/C Base Pair

PDB ID 1p0u

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