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1ks5

From Proteopedia

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(New page: 200px<br /><applet load="1ks5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ks5, resolution 2.1&Aring;" /> '''Structure of Aspergil...)
Current revision (08:03, 3 April 2024) (edit) (undo)
 
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[[Image:1ks5.gif|left|200px]]<br /><applet load="1ks5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ks5, resolution 2.1&Aring;" />
 
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'''Structure of Aspergillus niger endoglucanase'''<br />
 
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==Overview==
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==Structure of Aspergillus niger endoglucanase==
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The fungus Aspergillus niger is a main source of industrial cellulase., beta-1,4-Endoglucanase is the major component of cellulase from A. niger., In spite of widespread applications, little is known about the structure, of this enzyme. Here, the structure of beta-1,4-endoglucanase from A., niger (EglA) was determined at 2.1 A resolution. Although there is a low, sequence identity between EglA and CelB2, another member of family 12, the, three-dimensional structures of their core regions are quite similar. The, structural differences are mostly found in the loop regions, where CelB2, has an extra beta-sheet (beta-sheet C) at the non-reducing end of the, binding cleft of the native enzyme. Incubation of EglA with PdCl(2), irreversibly inhibits the EglA activity. Structural studies of the, enzyme-palladium complex show that three Pd(2+) ions bind to each EglA, molecule. One of the Pd(2+) ions forms a coordinate covalent bond with, Met118 S(delta) and the nucleophilic Glu116 O(epsilon1) at the active site, of the enzyme. The other two Pd(2+) ions bind on the surface of the, protein. Binding of Pd(2+) ions to EglA does not change the general, conformation of the backbone of the protein significantly. Based on this, structural study, one can conclude that the palladium ion directly binds, to and blocks the active site of EglA and thus inactivates the enzyme.
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<StructureSection load='1ks5' size='340' side='right'caption='[[1ks5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ks5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KS5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ks5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ks5 OCA], [https://pdbe.org/1ks5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ks5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ks5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ks5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O74705_ASPNG O74705_ASPNG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/1ks5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ks5 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KS5 OCA].
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Determination of the structure of an endoglucanase from Aspergillus niger and its mode of inhibition by palladium chloride., Khademi S, Zhang D, Swanson SM, Wartenberg A, Witte K, Meyer EF, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):660-7. Epub 2002, Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11914491 11914491]
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[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Cellulase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Khademi S]]
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[[Category: Khademi, S.]]
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[[Category: Meyer EF]]
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[[Category: Meyer, E. F.]]
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[[Category: Swanson SM]]
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[[Category: Swanson, S. M.]]
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[[Category: Wartenberg A]]
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[[Category: Wartenberg, A.]]
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[[Category: Witte C]]
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[[Category: Witte, C.]]
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[[Category: Zhang D]]
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[[Category: Zhang, D.]]
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[[Category: aspergillus niger]]
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[[Category: cellulase]]
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[[Category: endoglucanase]]
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[[Category: familly 12]]
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[[Category: glycosyl hydrolase]]
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[[Category: jelly-roll]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:50:44 2007''
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Current revision

Structure of Aspergillus niger endoglucanase

PDB ID 1ks5

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