1xcc

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(New page: 200px<br /><applet load="1xcc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xcc, resolution 2.30&Aring;" /> '''1-Cys peroxidoxin fr...)
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[[Image:1xcc.jpg|left|200px]]<br /><applet load="1xcc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xcc, resolution 2.30&Aring;" />
 
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'''1-Cys peroxidoxin from Plasmodium Yoelli'''<br />
 
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==Overview==
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==1-Cys peroxidoxin from Plasmodium Yoelli==
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Parasites from the protozoan phylum Apicomplexa are responsible for, diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of, which have significantly higher rates of mortality and morbidity in, economically underdeveloped regions of the world. Advances in vaccine, development and drug discovery are urgently needed to control these, diseases and can be facilitated by production of purified recombinant, proteins from Apicomplexan genomes and determination of their 3D, structures. To date, both heterologous expression and crystallization of, Apicomplexan proteins have seen only limited success. In an effort to, explore the effectiveness of producing and crystallizing proteins on a, genome-scale using a standardized methodology, over 400 distinct, Plasmodium falciparum target genes were chosen representing different, cellular classes, along with select orthologues from four other Plasmodium, species as well as Cryptosporidium parvum and Toxoplasma gondii. From a, total of 1008 genes from the seven genomes, 304 (30.2%) produced purified, soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal, structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for, genome-scale production and crystallography of Apicomplexan proteins., Predictably, orthologous proteins from different Apicomplexan genomes, behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ, significantly. Their differences were effectively exploited to elevate the, overall productivity to levels comparable to the most successful ongoing, structural genomics projects: 229 of the 468 target genes produced, purified soluble protein from one or more organisms, with 80 and 32 of the, purified targets, respectively, leading to crystals and ultimately, structures from one or more orthologues.
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<StructureSection load='1xcc' size='340' side='right'caption='[[1xcc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xcc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_yoelii Plasmodium yoelii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcc OCA], [https://pdbe.org/1xcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcc RCSB], [https://www.ebi.ac.uk/pdbsum/1xcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q86SB3_PLAYE Q86SB3_PLAYE] Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.[PIRNR:PIRNR000239]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XCC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_yoelii Plasmodium yoelii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XCC OCA].
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*[[Peroxiredoxin 3D structures|Peroxiredoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms., Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R, Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17125854 17125854]
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[[Category: Large Structures]]
[[Category: Plasmodium yoelii]]
[[Category: Plasmodium yoelii]]
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[[Category: Single protein]]
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[[Category: Amani M]]
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[[Category: Amani, M.]]
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[[Category: Arrowsmith C]]
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[[Category: Arrowsmith, C.]]
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[[Category: Bochkarev A]]
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[[Category: Bochkarev, A.]]
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[[Category: Bray J]]
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[[Category: Bray, J.]]
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[[Category: Edwards A]]
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[[Category: Edwards, A.]]
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[[Category: Houston S]]
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[[Category: Houston, S.]]
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[[Category: Hui R]]
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[[Category: Hui, R.]]
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[[Category: Lew J]]
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[[Category: Lew, J.]]
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[[Category: Sharma S]]
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[[Category: SGC, Structural.Genomics.Consortium.]]
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[[Category: Sundstrom M]]
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[[Category: Sharma, S.]]
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[[Category: Vedadi M]]
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[[Category: Sundstrom, M.]]
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[[Category: Wasney G]]
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[[Category: Vedadi, M.]]
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[[Category: Xu X]]
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[[Category: Wasney, G.]]
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[[Category: Xu, X.]]
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[[Category: sgc]]
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[[Category: structural genomics]]
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[[Category: structural genomics consortium]]
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[[Category: unknown function]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:50:50 2007''
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Current revision

1-Cys peroxidoxin from Plasmodium Yoelli

PDB ID 1xcc

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OCA

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