3hi8

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[[Image:3hi8.jpg|left|200px]]
 
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==Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii==
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The line below this paragraph, containing "STRUCTURE_3hi8", creates the "Structure Box" on the page.
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<StructureSection load='3hi8' size='340' side='right'caption='[[3hi8]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hi8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloferax_volcanii Haloferax volcanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HI8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.202&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hi8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi8 OCA], [https://pdbe.org/3hi8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hi8 RCSB], [https://www.ebi.ac.uk/pdbsum/3hi8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hi8 ProSAT]</span></td></tr>
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{{STRUCTURE_3hi8| PDB=3hi8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0VWY8_HALVO D0VWY8_HALVO] Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication (By similarity).[HAMAP-Rule:MF_00317]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hi8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hi8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The sliding clamp proliferating cell nuclear antigen (PCNA) plays vital roles in many aspects of DNA replication and repair in eukaryotic cells and in archaea. Realising the full potential of archaea as a model for PCNA function requires a combination of biochemical and genetic approaches. In order to provide a platform for subsequent reverse genetic analysis, PCNA from the halophilic archaeon Haloferax volcanii was subjected to crystallographic analysis. The gene was cloned and expressed in Escherichia coli and the protein was purified by affinity chromatography and crystallized by the vapour-diffusion technique. The structure was determined by molecular replacement and refined at 3.5 A resolution to a final R factor of 23.7% (R(free) = 25%). PCNA from H. volcanii was found to be homotrimeric and to resemble other homotrimeric PCNA clamps but with several differences that appear to be associated with adaptation of the protein to the high intracellular salt concentrations found in H. volcanii cells.
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===Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii===
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Structural insights into the adaptation of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii to a high-salt environment.,Morgunova E, Gray FC, Macneill SA, Ladenstein R Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1081-8. Epub 2009, Sep 16. PMID:19770505<ref>PMID:19770505</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hi8" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19770505}}, adds the Publication Abstract to the page
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*[[Proliferating cell nuclear antigen 3D structures|Proliferating cell nuclear antigen 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19770505 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19770505}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3HI8 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Haloferax_volcanii Haloferax volcanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI8 OCA].
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==Reference==
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<ref group="xtra">PMID:19770505</ref><references group="xtra"/>
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[[Category: Haloferax volcanii]]
[[Category: Haloferax volcanii]]
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[[Category: Gray, F C.]]
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[[Category: Large Structures]]
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[[Category: Ladenstein, R.]]
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[[Category: Gray FC]]
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[[Category: Morgunova, E.]]
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[[Category: Ladenstein R]]
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[[Category: S A., MacNeill.]]
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[[Category: MacNeill SA]]
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[[Category: Dna interaction]]
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[[Category: Morgunova E]]
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[[Category: Haloferax volcanii]]
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[[Category: Replication]]
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[[Category: Sliding clamp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 30 09:14:05 2009''
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Current revision

Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii

PDB ID 3hi8

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