1ku2

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(New page: 200px<br /><applet load="1ku2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ku2, resolution 2.9&Aring;" /> '''Crystal Structure of ...)
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[[Image:1ku2.gif|left|200px]]<br /><applet load="1ku2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ku2, resolution 2.9&Aring;" />
 
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'''Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment Containing Regions 1.2 to 3.1'''<br />
 
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==Overview==
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==Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment Containing Regions 1.2 to 3.1==
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The sigma subunit is the key regulator of bacterial transcription., Proteolysis of Thermus aquaticus sigma(A), which occurred in situ during, crystallization, reveals three domains, sigma(2), sigma(3), and sigma(4), connected by flexible linkers. Crystal structures of each domain were, determined, as well as of sigma(4) complexed with -35 element DNA. Exposed, surfaces of each domain are important for RNA polymerase binding., Universally conserved residues important for -10 element recognition and, melting lie on one face of sigma(2), while residues important for extended, -10 recognition lie on sigma(3). Genetic studies correctly predicted that, a helix-turn-helix motif in sigma(4) recognizes the -35 element but not, the details of the protein-DNA interactions. Positive control mutants in, sigma(4) cluster in two regions, positioned to interact with activators, bound just upstream or downstream of the -35 element.
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<StructureSection load='1ku2' size='340' side='right'caption='[[1ku2]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ku2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KU2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ku2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ku2 OCA], [https://pdbe.org/1ku2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ku2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ku2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ku2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIGA_THEAQ SIGA_THEAQ] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.[HAMAP-Rule:MF_00963]<ref>PMID:11114902</ref> <ref>PMID:11931761</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/1ku2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ku2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KU2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KU2 OCA].
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the bacterial RNA polymerase promoter specificity sigma subunit., Campbell EA, Muzzin O, Chlenov M, Sun JL, Olson CA, Weinman O, Trester-Zedlitz ML, Darst SA, Mol Cell. 2002 Mar;9(3):527-39. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11931761 11931761]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Campbell, E.A.]]
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[[Category: Campbell EA]]
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[[Category: Chlenov, M.]]
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[[Category: Chlenov M]]
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[[Category: Darst, S.A.]]
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[[Category: Darst SA]]
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[[Category: Muzzin, O.]]
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[[Category: Muzzin O]]
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[[Category: Olson, C.A.]]
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[[Category: Olson CA]]
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[[Category: Sun, J.L.]]
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[[Category: Sun JL]]
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[[Category: Trester-Zedlitz, M.L.]]
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[[Category: Trester-Zedlitz ML]]
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[[Category: Weinman, O.]]
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[[Category: Weinman O]]
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[[Category: SO4]]
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[[Category: helices]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:56:52 2007''
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Current revision

Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment Containing Regions 1.2 to 3.1

PDB ID 1ku2

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