1p4t

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(New page: 200px<br /><applet load="1p4t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p4t, resolution 2.55&Aring;" /> '''Crystal structure of...)
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[[Image:1p4t.jpg|left|200px]]<br /><applet load="1p4t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p4t, resolution 2.55&Aring;" />
 
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'''Crystal structure of Neisserial surface protein A (NspA)'''<br />
 
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==Overview==
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==Crystal structure of Neisserial surface protein A (NspA)==
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The neisserial surface protein A (NspA) from Neisseria meningitidis is a, promising vaccine candidate because it is highly conserved among, meningococcal strains and induces bactericidal antibodies. NspA is a, homolog of the Opa proteins, which mediate adhesion to host cells. Here, we present the crystal structure of NspA, determined to 2.55-A resolution., NspA forms an eight-stranded antiparallel beta-barrel. The four loops at, the extracellular side of the NspA molecule form a long cleft, which, contains mainly hydrophobic residues and harbors a detergent molecule, suggesting that the protein might function in the binding of hydrophobic, ligands, such as lipids. In addition, the structure provides a starting, point for structure-based vaccine design.
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<StructureSection load='1p4t' size='340' side='right'caption='[[1p4t]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p4t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P4T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CXE:PENTAETHYLENE+GLYCOL+MONODECYL+ETHER'>CXE</scene>, <scene name='pdbligand=ETA:ETHANOLAMINE'>ETA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4t OCA], [https://pdbe.org/1p4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p4t RCSB], [https://www.ebi.ac.uk/pdbsum/1p4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p4t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RP17_NEIME Q9RP17_NEIME]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/1p4t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p4t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The neisserial surface protein A (NspA) from Neisseria meningitidis is a promising vaccine candidate because it is highly conserved among meningococcal strains and induces bactericidal antibodies. NspA is a homolog of the Opa proteins, which mediate adhesion to host cells. Here, we present the crystal structure of NspA, determined to 2.55-A resolution. NspA forms an eight-stranded antiparallel beta-barrel. The four loops at the extracellular side of the NspA molecule form a long cleft, which contains mainly hydrophobic residues and harbors a detergent molecule, suggesting that the protein might function in the binding of hydrophobic ligands, such as lipids. In addition, the structure provides a starting point for structure-based vaccine design.
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==About this Structure==
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Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential.,Vandeputte-Rutten L, Bos MP, Tommassen J, Gros P J Biol Chem. 2003 Jul 4;278(27):24825-30. Epub 2003 Apr 26. PMID:12716881<ref>PMID:12716881</ref>
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1P4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis] with SO4, CXE and ETA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P4T OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential., Vandeputte-Rutten L, Bos MP, Tommassen J, Gros P, J Biol Chem. 2003 Jul 4;278(27):24825-30. Epub 2003 Apr 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12716881 12716881]
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</div>
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<div class="pdbe-citations 1p4t" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Neisseria meningitidis]]
[[Category: Neisseria meningitidis]]
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[[Category: Single protein]]
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[[Category: Bos MP]]
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[[Category: Bos, M.P.]]
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[[Category: Gros P]]
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[[Category: Gros, P.]]
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[[Category: Tommassen J]]
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[[Category: Tommassen, J.]]
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[[Category: Vandeputte-Rutten L]]
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[[Category: Vandeputte-Rutten, L.]]
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[[Category: CXE]]
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[[Category: ETA]]
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[[Category: SO4]]
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[[Category: beta barrel]]
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[[Category: outer membrane protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:58:57 2007''
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Current revision

Crystal structure of Neisserial surface protein A (NspA)

PDB ID 1p4t

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