2w2h

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{{Seed}}
 
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[[Image:2w2h.png|left|200px]]
 
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==Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV==
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The line below this paragraph, containing "STRUCTURE_2w2h", creates the "Structure Box" on the page.
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<StructureSection load='2w2h' size='340' side='right'caption='[[2w2h]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w2h]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus] and [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_2w2h| PDB=2w2h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2h OCA], [https://pdbe.org/2w2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2h RCSB], [https://www.ebi.ac.uk/pdbsum/2w2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CCNT1_HORSE CCNT1_HORSE] Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II.[UniProtKB:O60563]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w2h ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The replication of many retroviruses is mediated by a transcriptional activator protein, Tat, which activates RNA polymerase II at the level of transcription elongation. Tat interacts with Cyclin T1 of the positive transcription-elongation factor P-TEFb to recruit the transactivation-response TAR RNA, which acts as a promoter element in the transcribed 5' end of the viral long terminal repeat. Here we present the structure of the cyclin box domain of Cyclin T1 in complex with the Tat protein from the equine infectious anemia virus and its corresponding TAR RNA. The basic RNA-recognition motif of Tat adopts a helical structure whose flanking regions interact with a cyclin T-specific loop in the first cyclin box repeat. Together, both proteins coordinate the stem-loop structure of TAR. Our findings show that Tat binds to a surface on Cyclin T1 similar to where recognition motifs from substrate and inhibitor peptides were previously found to interact within Cdk-cyclin pairs.
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===STRUCTURAL BASIS OF TRANSCRIPTION ACTIVATION BY THE CYCLIN T1-TAT-TAR RNA COMPLEX FROM EIAV===
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Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV.,Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M Nat Struct Mol Biol. 2008 Dec;15(12):1287-92. Epub 2008 Nov 23. PMID:19029897<ref>PMID:19029897</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2w2h" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19029897}}, adds the Publication Abstract to the page
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*[[Cyclin 3D structures|Cyclin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19029897 is the PubMed ID number.
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*[[Tat protein|Tat protein]]
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== References ==
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{{ABSTRACT_PUBMED_19029897}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2W2H is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Equine_infectious_anemia_virus Equine infectious anemia virus] and [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2H OCA].
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==Reference==
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<ref group="xtra">PMID:19029897</ref><references group="xtra"/>
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[[Category: Equine infectious anemia virus]]
[[Category: Equine infectious anemia virus]]
[[Category: Equus caballus]]
[[Category: Equus caballus]]
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[[Category: Anand, K.]]
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[[Category: Large Structures]]
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[[Category: Geyer, M.]]
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[[Category: Anand K]]
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[[Category: Acetylation]]
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[[Category: Geyer M]]
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[[Category: Activator]]
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[[Category: Cell cycle]]
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[[Category: Cell division]]
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[[Category: Coiled coil]]
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[[Category: Crystallization]]
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[[Category: Cyclin]]
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[[Category: Cyclin t1]]
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[[Category: Host-virus interaction]]
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[[Category: Nucleus]]
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[[Category: Rna-binding]]
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[[Category: Rna-binding protein]]
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[[Category: Tar]]
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[[Category: Tat]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 7 13:32:42 2009''
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Current revision

Structural basis of transcription activation by the Cyclin T1-Tat-TAR RNA complex from EIAV

PDB ID 2w2h

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