1gzj

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(New page: 200px<br /><applet load="1gzj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gzj, resolution 1.62&Aring;" /> '''STRUCTURE OF THERMOA...)
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[[Image:1gzj.jpg|left|200px]]<br /><applet load="1gzj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gzj, resolution 1.62&Aring;" />
 
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'''STRUCTURE OF THERMOASCUS AURANTIACUS FAMILY 5 ENDOGLUCANASE'''<br />
 
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==Overview==
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==Structure of Thermoascus aurantiacus family 5 endoglucanase==
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The crystal structure of Thermoascus aurantiacus endoglucanase (Cel5A), a, family 5 glycoside hydrolase, has been determined to 1.62 A resolution by, multiple isomorphous replacement with anomalous scattering. It is the, first report of a structure in the subfamily to which Cel5A belongs. Cel5A, consists solely of a catalytic module with compact eight-fold beta/alpha, barrel architecture. The length of the tryptophan-rich substrate binding, groove suggests the presence of substrate binding subsites -4 to +3., Structural comparison shows that two glycines are completely conserved in, the family, in addition to the two catalytic glutamates and six other, conserved residues previously identified. Gly 44 in particular is part of, a type IV C-terminal helix capping motif, whose disruption is likely to, affect the position of an essential conserved arginine. One aromatic, residue (Trp 170 in Cel5A), not conserved in term of sequence, is, nonetheless spatially conserved in the substrate binding groove. Its role, might be to force the bend that occurs in the polysaccharide chain on, binding, thus favoring substrate distortion at subsite -1.
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<StructureSection load='1gzj' size='340' side='right'caption='[[1gzj]], [[Resolution|resolution]] 1.62&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gzj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.62&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gzj OCA], [https://pdbe.org/1gzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gzj RCSB], [https://www.ebi.ac.uk/pdbsum/1gzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gzj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8TG26_THEAU Q8TG26_THEAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gzj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gzj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of Thermoascus aurantiacus endoglucanase (Cel5A), a family 5 glycoside hydrolase, has been determined to 1.62 A resolution by multiple isomorphous replacement with anomalous scattering. It is the first report of a structure in the subfamily to which Cel5A belongs. Cel5A consists solely of a catalytic module with compact eight-fold beta/alpha barrel architecture. The length of the tryptophan-rich substrate binding groove suggests the presence of substrate binding subsites -4 to +3. Structural comparison shows that two glycines are completely conserved in the family, in addition to the two catalytic glutamates and six other conserved residues previously identified. Gly 44 in particular is part of a type IV C-terminal helix capping motif, whose disruption is likely to affect the position of an essential conserved arginine. One aromatic residue (Trp 170 in Cel5A), not conserved in term of sequence, is nonetheless spatially conserved in the substrate binding groove. Its role might be to force the bend that occurs in the polysaccharide chain on binding, thus favoring substrate distortion at subsite -1.
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==About this Structure==
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The 1.62 A structure of Thermoascus aurantiacus endoglucanase: completing the structural picture of subfamilies in glycoside hydrolase family 5.,Lo Leggio L, Larsen S FEBS Lett. 2002 Jul 17;523(1-3):103-8. PMID:12123813<ref>PMID:12123813</ref>
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1GZJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoascus_aurantiacus Thermoascus aurantiacus]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The 1.62 A structure of Thermoascus aurantiacus endoglucanase: completing the structural picture of subfamilies in glycoside hydrolase family 5., Lo Leggio L, Larsen S, FEBS Lett. 2002 Jul 17;523(1-3):103-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12123813 12123813]
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</div>
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[[Category: Cellulase]]
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<div class="pdbe-citations 1gzj" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Thermoascus aurantiacus]]
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[[Category: Larsen, S.]]
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[[Category: Leggio, L.Lo.]]
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[[Category: Pickersgill, R.W.]]
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[[Category: family 5]]
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[[Category: glycoside hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:01:20 2007''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermoascus aurantiacus]]
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[[Category: Larsen S]]
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[[Category: Lo Leggio L]]
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[[Category: Pickersgill RW]]

Current revision

Structure of Thermoascus aurantiacus family 5 endoglucanase

PDB ID 1gzj

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