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1tet

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(New page: 200px<br /><applet load="1tet" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tet, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1tet.gif|left|200px]]<br /><applet load="1tet" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tet, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS==
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Cholera toxin peptide 3 (CTP3) is a 15-residue peptide corresponding in, sequence to an immunogenic loop on the surface of the B-subunits of both, cholera toxin and the heat-labile toxin from Escherichia coli. TE33 is the, Fab fragment of a monoclonal antibody elicited against CTP3. The crystal, structure of the TE33-CTP3 complex at 2.3 A resolution reveals an, antigen-binding pocket, 13 A deep and 13 A wide, which is lined with many, aromatic residues. The N-terminal portion of the peptide antigen CTP3, forms a type II beta-turn that fits snugly into this pocket. At gln7 the, peptide backbone of CTP3 forms a kink followed by an extended C-terminal, chain that seals off the cleft and buries the beta-turn underneath it. All, six complementarity-determining regions of TE33 contribute to the binding, of CTP3. The antibody-peptide contacts include, in addition to van der, Waals' interactions and hydrogen bonds, also one salt bridge and one water, molecule, which mediates the interaction.
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<StructureSection load='1tet' size='340' side='right'caption='[[1tet]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tet]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TET FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tet OCA], [https://pdbe.org/1tet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tet RCSB], [https://www.ebi.ac.uk/pdbsum/1tet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tet ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ELBP_ECOLX ELBP_ECOLX] The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tet_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tet ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TET is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TET OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[User:David Solfiell/sandbox 1|User:David Solfiell/sandbox 1]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Crystal structure of an anticholera toxin peptide complex at 2.3 A., Shoham M, J Mol Biol. 1993 Aug 20;232(4):1169-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7690406 7690406]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Shoham M]]
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[[Category: Shoham, M.]]
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[[Category: CIT]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:06:04 2007''
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Current revision

CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS

PDB ID 1tet

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