We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1tet

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1tet" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tet, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Current revision (00:31, 21 November 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tet.gif|left|200px]]<br /><applet load="1tet" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1tet, resolution 2.3&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS==
-
Cholera toxin peptide 3 (CTP3) is a 15-residue peptide corresponding in, sequence to an immunogenic loop on the surface of the B-subunits of both, cholera toxin and the heat-labile toxin from Escherichia coli. TE33 is the, Fab fragment of a monoclonal antibody elicited against CTP3. The crystal, structure of the TE33-CTP3 complex at 2.3 A resolution reveals an, antigen-binding pocket, 13 A deep and 13 A wide, which is lined with many, aromatic residues. The N-terminal portion of the peptide antigen CTP3, forms a type II beta-turn that fits snugly into this pocket. At gln7 the, peptide backbone of CTP3 forms a kink followed by an extended C-terminal, chain that seals off the cleft and buries the beta-turn underneath it. All, six complementarity-determining regions of TE33 contribute to the binding, of CTP3. The antibody-peptide contacts include, in addition to van der, Waals' interactions and hydrogen bonds, also one salt bridge and one water, molecule, which mediates the interaction.
+
<StructureSection load='1tet' size='340' side='right'caption='[[1tet]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1tet]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TET FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tet OCA], [https://pdbe.org/1tet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tet RCSB], [https://www.ebi.ac.uk/pdbsum/1tet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tet ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ELBP_ECOLX ELBP_ECOLX] The biological activity of the toxin is produced by the A chain, which activates intracellular adenyl cyclase.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tet_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tet ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Cholera toxin peptide 3 (CTP3) is a 15-residue peptide corresponding in sequence to an immunogenic loop on the surface of the B-subunits of both cholera toxin and the heat-labile toxin from Escherichia coli. TE33 is the Fab fragment of a monoclonal antibody elicited against CTP3. The crystal structure of the TE33-CTP3 complex at 2.3 A resolution reveals an antigen-binding pocket, 13 A deep and 13 A wide, which is lined with many aromatic residues. The N-terminal portion of the peptide antigen CTP3 forms a type II beta-turn that fits snugly into this pocket. At gln7 the peptide backbone of CTP3 forms a kink followed by an extended C-terminal chain that seals off the cleft and buries the beta-turn underneath it. All six complementarity-determining regions of TE33 contribute to the binding of CTP3. The antibody-peptide contacts include, in addition to van der Waals' interactions and hydrogen bonds, also one salt bridge and one water molecule, which mediates the interaction.
-
==About this Structure==
+
Crystal structure of an anticholera toxin peptide complex at 2.3 A.,Shoham M J Mol Biol. 1993 Aug 20;232(4):1169-75. PMID:7690406<ref>PMID:7690406</ref>
-
1TET is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TET OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of an anticholera toxin peptide complex at 2.3 A., Shoham M, J Mol Biol. 1993 Aug 20;232(4):1169-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7690406 7690406]
+
</div>
-
[[Category: Mus musculus]]
+
<div class="pdbe-citations 1tet" style="background-color:#fffaf0;"></div>
-
[[Category: Protein complex]]
+
-
[[Category: Shoham, M.]]
+
-
[[Category: CIT]]
+
-
[[Category: immunoglobulin]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:06:04 2007''
+
==See Also==
 +
*[[Antibody 3D structures|Antibody 3D structures]]
 +
*[[User:David Solfiell/sandbox 1|User:David Solfiell/sandbox 1]]
 +
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Mus musculus]]
 +
[[Category: Shoham M]]

Current revision

CRYSTAL STRUCTURE OF AN ANTICHOLERA TOXIN PEPTIDE COMPLEX AT 2.3 ANGSTROMS

PDB ID 1tet

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tet"
Personal tools