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1m85

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[[Image:1m85.gif|left|200px]]
 
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<applet load="1m85" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1m85, resolution 2.00&Aring;" />
 
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'''Structure of Proteus mirabilis catalase for the native form'''<br />
 
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==Overview==
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==Structure of Proteus mirabilis catalase for the native form==
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A catalase from a peroxide resistant mutant of Proteus mirabilis binds, NADPH tightly. Interestingly, this enzyme can be stripped of NADPH without, loss of the catalatic activity. It is the only known non-mammalian, catalase able to bind NADPH. The structure without cofactor was solved by, molecular replacement using the structure of beef liver catalase as a, model. The structure was refined to an R-factor of 19.3% in the range 8 to, 2.2 A resolution. According to the sequence, a methionine sulphone was, positioned in the haem active site. This oxidized form of methionine is, particular to Proteus mirabilis catalase and likely to produce some steric, hindrance in the active site. Two important water molecules are positioned, in the haem distal site. These two water molecules are not ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7791219 (full description)]]
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<StructureSection load='1m85' size='340' side='right'caption='[[1m85]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2cae 2cae] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cae 1cae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M85 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OMT:S-DIOXYMETHIONINE'>OMT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m85 OCA], [https://pdbe.org/1m85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m85 RCSB], [https://www.ebi.ac.uk/pdbsum/1m85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m85 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CATA_PROMI CATA_PROMI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m85_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m85 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1M85 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis]] with SO4, HEM and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entries 2CAE and 1CAE.
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*[[Catalase 3D structures|Catalase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of Proteus mirabilis PR catalase with and without bound NADPH., Gouet P, Jouve HM, Dideberg O, J Mol Biol. 1995 Jun 23;249(5):933-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7791219 7791219]
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[[Category: Large Structures]]
[[Category: Proteus mirabilis]]
[[Category: Proteus mirabilis]]
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[[Category: Single protein]]
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[[Category: Dideberg O]]
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[[Category: Dideberg, O.]]
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[[Category: Gouet P]]
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[[Category: Gouet, P.]]
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[[Category: Jouve H-M]]
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[[Category: Jouve, H.M.]]
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[[Category: GOL]]
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[[Category: HEM]]
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[[Category: SO4]]
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[[Category: methionine sulfone]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Oct 28 18:00:39 2007''
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Current revision

Structure of Proteus mirabilis catalase for the native form

PDB ID 1m85

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