2kj4

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{{Seed}}
 
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[[Image:2kj4.jpg|left|200px]]
 
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==Solution structure of the complex of VEK-30 and plasminogen kringle 2==
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The line below this paragraph, containing "STRUCTURE_2kj4", creates the "Structure Box" on the page.
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<StructureSection load='2kj4' size='340' side='right'caption='[[2kj4]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kj4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KJ4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kj4 OCA], [https://pdbe.org/2kj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kj4 RCSB], [https://www.ebi.ac.uk/pdbsum/2kj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kj4 ProSAT]</span></td></tr>
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{{STRUCTURE_2kj4| PDB=2kj4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6V4L8_STRPY Q6V4L8_STRPY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/2kj4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kj4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of the complex containing the isolated kringle 2 domain of human plasminogen (K2(Pg)) and VEK-30, a 30-amino acid residue internal peptide from a streptococcal M-like plasminogen (Pg) binding protein (PAM), has been determined by multinuclear high-resolution NMR. Complete backbone and side-chain assignments were obtained from triple-resonance experiments, after which structure calculations were performed and ultimately refined by restrained molecular simulation in water. We find that, in contrast with the dimer of complexes observed in the asymmetric unit of the crystal, global correlation times and buoyant molecular weight determinations of the complex and its individual components showed the monomeric nature of all species in solution. The NMR-derived structure of K2(Pg) in complex with VEK-30 presents a folding pattern typical of other kringle domains, while bound VEK-30 forms an end-to-end alpha-helix (residues 6-27) in the complex. Most of the VEK-30/K2(Pg) interactions in solution occur between a single face of the alpha-helix of VEK-30 and the lysine binding site (LBS) of K2(Pg). The canonical LBS of K2(Pg), consisting of Asp54, Asp56, Trp60, Arg69, and Trp70 (kringle numbering), interacts with an internal pseudo-lysine of VEK-30, comprising side-chains of Arg17, His18, and Glu20. Site-specific mutagenesis analysis confirmed that the electrostatic field formed by the N-terminal anionic residues of the VEK-30 alpha-helix, viz., Asp7, and the non-conserved cationic residues of K2(Pg), viz., Lys43 and Arg55, play additional important roles in the docking of VEK-30 to K2(Pg). Structural analysis and kringle sequence alignments revealed several important features related to exosite binding that provide a structural rationale for the high specificity and affinity of VEK-30 for K2(Pg).
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===Solution structure of the complex of VEK-30 and plasminogen kringle 2===
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Solution structure of the complex of VEK-30 and plasminogen kringle 2.,Wang M, Zajicek J, Geiger JH, Prorok M, Castellino FJ J Struct Biol. 2010 Mar;169(3):349-59. Epub 2009 Sep 30. PMID:19800007<ref>PMID:19800007</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2kj4" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19800007}}, adds the Publication Abstract to the page
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*[[Plasminogen 3D structures|Plasminogen 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19800007 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19800007}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2KJ4 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJ4 OCA].
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==Reference==
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<ref group="xtra">PMID:19800007</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Streptococcus pyogenes]]
[[Category: Streptococcus pyogenes]]
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[[Category: Castellin, F J.]]
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[[Category: Castellin FJ]]
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[[Category: Prorok, M.]]
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[[Category: Prorok M]]
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[[Category: Wang, M.]]
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[[Category: Wang M]]
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[[Category: Zajicek, J.]]
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[[Category: Zajicek J]]
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[[Category: Blood clotting]]
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[[Category: Protein/peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 21 10:26:31 2009''
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Current revision

Solution structure of the complex of VEK-30 and plasminogen kringle 2

PDB ID 2kj4

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