1tqp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1tqp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tqp, resolution 2.10&Aring;" /> '''Crystal Structure of...)
Current revision (07:29, 30 October 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tqp.gif|left|200px]]<br /><applet load="1tqp" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1tqp, resolution 2.10&Aring;" />
 
-
'''Crystal Structure of A. fulgidus Rio2 Serine Protein Kinase Bound to ATP'''<br />
 
-
==Overview==
+
==Crystal Structure of A. fulgidus Rio2 Serine Protein Kinase Bound to ATP==
-
The RIO family of atypical serine/threonine kinases contains two, subfamilies, Rio1 and Rio2, highly conserved from archaea to man. Both RIO, proteins from Saccharomyces cerevisiae catalyze serine phosphorylation in, vitro, and the presence of conserved catalytic residues is required for, cell viability. The activity of Rio2 is necessary for rRNA cleavage in 40S, ribosomal subunit maturation. We solved the X-ray crystal structure of, Archaeoglobus fulgidus Rio2, with and without bound nucleotides, at 2.0 A, resolution. The C-terminal RIO domain is indeed structurally homologous to, protein kinases, although it differs from known serine kinases in ATP, binding and lacks the regions important for substrate binding., Unexpectedly, the N-terminal Rio2-specific domain contains a winged helix, fold, seen primarily in DNA-binding proteins. These discoveries have, implications in determining the target and function of RIO proteins and, define a distinct new family of protein kinases.
+
<StructureSection load='1tqp' size='340' side='right'caption='[[1tqp]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1tqp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TQP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tqp OCA], [https://pdbe.org/1tqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tqp RCSB], [https://www.ebi.ac.uk/pdbsum/1tqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tqp ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RIO2_ARCFU RIO2_ARCFU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tq/1tqp_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tqp ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The RIO family of atypical serine/threonine kinases contains two subfamilies, Rio1 and Rio2, highly conserved from archaea to man. Both RIO proteins from Saccharomyces cerevisiae catalyze serine phosphorylation in vitro, and the presence of conserved catalytic residues is required for cell viability. The activity of Rio2 is necessary for rRNA cleavage in 40S ribosomal subunit maturation. We solved the X-ray crystal structure of Archaeoglobus fulgidus Rio2, with and without bound nucleotides, at 2.0 A resolution. The C-terminal RIO domain is indeed structurally homologous to protein kinases, although it differs from known serine kinases in ATP binding and lacks the regions important for substrate binding. Unexpectedly, the N-terminal Rio2-specific domain contains a winged helix fold, seen primarily in DNA-binding proteins. These discoveries have implications in determining the target and function of RIO proteins and define a distinct new family of protein kinases.
-
==About this Structure==
+
Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases.,LaRonde-LeBlanc N, Wlodawer A Structure. 2004 Sep;12(9):1585-94. PMID:15341724<ref>PMID:15341724</ref>
-
1TQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304] with ATP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TQP OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases., LaRonde-LeBlanc N, Wlodawer A, Structure. 2004 Sep;12(9):1585-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341724 15341724]
+
</div>
-
[[Category: Archaeoglobus fulgidus dsm 4304]]
+
<div class="pdbe-citations 1tqp" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: LaRonde-LeBlanc, N.]]
+
-
[[Category: Wlodawer, A.]]
+
-
[[Category: ATP]]
+
-
[[Category: atp]]
+
-
[[Category: serine kinase]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:35:06 2007''
+
==See Also==
 +
*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Archaeoglobus fulgidus DSM 4304]]
 +
[[Category: Large Structures]]
 +
[[Category: LaRonde-LeBlanc N]]
 +
[[Category: Wlodawer A]]

Current revision

Crystal Structure of A. fulgidus Rio2 Serine Protein Kinase Bound to ATP

PDB ID 1tqp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tqp"
Personal tools