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2ob7

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{{Seed}}
 
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[[Image:2ob7.png|left|200px]]
 
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==Structure of tmRNA-(SmpB)2 complex as inferred from cryo-EM==
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The line below this paragraph, containing "STRUCTURE_2ob7", creates the "Structure Box" on the page.
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<SX load='2ob7' size='340' side='right' viewer='molstar' caption='[[2ob7]], [[Resolution|resolution]] 13.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ob7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OB7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ob7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ob7 OCA], [https://pdbe.org/2ob7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ob7 RCSB], [https://www.ebi.ac.uk/pdbsum/2ob7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ob7 ProSAT]</span></td></tr>
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{{STRUCTURE_2ob7| PDB=2ob7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSRP_AQUAE SSRP_AQUAE] Binds specifically to the SsrA RNA (tmRNA) and is required for stable association of SsrA with ribosomes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/2ob7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ob7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A eubacterial ribosome stalled on a defective mRNA can be released through a quality control mechanism referred to as trans-translation, which depends on the coordinating binding actions of transfer-messenger RNA, small protein B, and ribosome protein S1. By means of cryo-electron microscopy, we obtained a map of the complex composed of a stalled ribosome and small protein B, which appears near the decoding center. This result suggests that, when lacking a codon, the A-site on the small subunit is a target for small protein B. To investigate the role of S1 played in trans-translation, we obtained a cryo-electron microscopic map, including a stalled ribosome, transfer-messenger RNA, and small protein Bs but in the absence of S1. In this complex, several connections between the 30 S subunit and transfer-messenger RNA that appear in the +S1 complex are no longer found. We propose the unifying concept of scaffolding for the roles of small protein B and S1 in binding of transfer-messenger RNA to the ribosome during trans-translation, and we infer a pathway of sequential binding events in the initial phase of trans-translation.
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===Structure of tmRNA-(SmpB)2 complex as inferred from cryo-EM===
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Scaffolding as an organizing principle in trans-translation. The roles of small protein B and ribosomal protein S1.,Gillet R, Kaur S, Li W, Hallier M, Felden B, Frank J J Biol Chem. 2007 Mar 2;282(9):6356-63. Epub 2006 Dec 19. PMID:17179154<ref>PMID:17179154</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17179154}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2ob7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17179154 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17179154}}
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__TOC__
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</SX>
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==About this Structure==
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[[Category: Large Structures]]
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2OB7 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OB7 OCA].
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==Reference==
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<ref group="xtra">PMID:17179154</ref><references group="xtra"/>
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Felden, B.]]
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[[Category: Felden B]]
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[[Category: Frank, J.]]
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[[Category: Frank J]]
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[[Category: Gillet, R.]]
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[[Category: Gillet R]]
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[[Category: Li, W.]]
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[[Category: Li W]]
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[[Category: Rna binding protein/rna complex]]
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[[Category: Smpb]]
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[[Category: Tmrna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 28 11:31:32 2009''
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Current revision

Structure of tmRNA-(SmpB)2 complex as inferred from cryo-EM

2ob7, resolution 13.60Å

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OCA

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