1d90

[[Image:1d90.gif|left|200px]]<br /><applet load="1d90" size="450" color="white" frame="true" align="right" spinBox="true"

caption="1d90, resolution 1.700&Aring;" />

'''REFINED CRYSTAL STRUCTURE OF AN OCTANUCLEOTIDE DUPLEX WITH I.T MISMATCHED BASE PAIRS'''<br />

==Overview==

The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been, determined by single crystal X-ray diffraction techniques to a resolution, of 1.7A. The sequence crystallises in space group P6(1), with unit cell, dimensions a = b = 45.07, c = 45.49A. The refinement converged with a, crystallographic residual R = 0.14 and the location of 81 solvent, molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C), base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure, shows it to be essentially isomorphous with that reported for d(GGGGCTCC), with the mispairs adopting a "wobble" conformation. Conformational, parameters and base stacking interactions are compared to those for the, native duplex d(GGGGCCCC) and other similar sequences. A rationale for the, apparent increased crystal packing efficiency and lattice stability of the, I.T octamer is given.

 

==About this Structure==

1D90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D90 OCA].

 

==Reference==

Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs., Cruse WB, Aymani J, Kennard O, Brown T, Jack AG, Leonard GA, Nucleic Acids Res. 1989 Jan 11;17(1):55-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2911488 2911488]

[[Category: Protein complex]]

[[Category: Aymani, J.]]

[[Category: Brown, T.]]

[[Category: Cruse, W.B.T.]]

[[Category: Jack, A.G.C.]]

[[Category: Kennard, O.]]

[[Category: Leonard, G.A.]]

[[Category: a-dna]]

[[Category: double helix]]

[[Category: mismatched]]

[[Category: modified]]