3i2q

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{{Seed}}
 
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[[Image:3i2q.jpg|left|200px]]
 
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==Crystal structure of the hairpin ribozyme with 2'OMe substrate strand and N1-deazaadenosine at position A9==
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The line below this paragraph, containing "STRUCTURE_3i2q", creates the "Structure Box" on the page.
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<StructureSection load='3i2q' size='340' side='right'caption='[[3i2q]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3i2q]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I2Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1DP:N1-DEAZA-ADENOSINE-5-MONOPHOSPHATE'>1DP</scene>, <scene name='pdbligand=A2M:2-O-METHYLADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>A2M</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene>, <scene name='pdbligand=S9L:2-[2-(2-HYDROXYETHOXY)ETHOXY]ETHYL+DIHYDROGEN+PHOSPHATE'>S9L</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3i2q| PDB=3i2q | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i2q OCA], [https://pdbe.org/3i2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i2q RCSB], [https://www.ebi.ac.uk/pdbsum/3i2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i2q ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hairpin ribozyme cleaves a phosphodiester bond within a cognate substrate. Structural and biochemical data indicate the conserved A9 and A10 bases reside close to the scissile bond but make distinct contributions to catalysis. To investigate these residues, we replaced the imino moiety of each base with N1-deazaadenosine. This single-atom change resulted in an 8-fold loss in k(obs) for A9 and displacement of the base from the active site; no effects were observed for A10. We propose that the imino moiety of A9 promotes a key water-mediated contact that favors transition-state formation, which suggests an enhanced chemical repertoire for RNA.
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===Crystal structure of the hairpin ribozyme with 2'OMe substrate strand and N1-deazaadenosine at position A9===
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Single-atom imino substitutions at A9 and A10 reveal distinct effects on the fold and function of the hairpin ribozyme catalytic core.,Spitale RC, Volpini R, Mungillo MV, Krucinska J, Cristalli G, Wedekind JE Biochemistry. 2009 Aug 25;48(33):7777-9. PMID:19634899<ref>PMID:19634899</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3i2q" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19634899}}, adds the Publication Abstract to the page
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19634899 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19634899}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3I2Q is a 3 chains structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I2Q OCA].
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[[Category: Krucinska J]]
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[[Category: Spitale RC]]
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==Reference==
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[[Category: Wedekind JE]]
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<ref group="xtra">PMID:19634899</ref><references group="xtra"/>
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[[Category: Krucinska, J.]]
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[[Category: Spitale, R C.]]
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[[Category: Wedekind, J E.]]
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[[Category: Hairpin ribozyme]]
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[[Category: N1-deazaadenosine]]
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[[Category: Rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 4 10:59:25 2009''
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Current revision

Crystal structure of the hairpin ribozyme with 2'OMe substrate strand and N1-deazaadenosine at position A9

PDB ID 3i2q

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