2wpo

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{{Seed}}
 
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[[Image:2wpo.png|left|200px]]
 
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==HCMV protease inhibitor complex==
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The line below this paragraph, containing "STRUCTURE_2wpo", creates the "Structure Box" on the page.
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<StructureSection load='2wpo' size='340' side='right'caption='[[2wpo]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wpo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betaherpesvirus_5 Human betaherpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WPO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=01E:(2S)-2-(3,3-DIMETHYLBUTANOYLAMINO)-N-[(2S)-1-[[(2S,3S)-3-HYDROXY-4-[(4-IODOPHENYL)METHYLAMINO]-4-OXO-BUTAN-2-YL]AMINO]-1,4-DIOXO-4-PYRROL-1-YL-BUTAN-2-YL]-3,3-DIMETHYL-BUTANAMIDE'>01E</scene></td></tr>
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{{STRUCTURE_2wpo| PDB=2wpo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wpo OCA], [https://pdbe.org/2wpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wpo RCSB], [https://www.ebi.ac.uk/pdbsum/2wpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wpo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SCAF_HCMVA SCAF_HCMVA] Capsid scaffolding protein acts as a scaffold protein by binding major capsid protein UL86 in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as protein UL86 forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein UL86. Cleavages products are evicted from the capsid before or during DNA packaging (By similarity). Assemblin is a protease essential for virion assembly in the nucleus. Catalyzes the cleavage of the assembly protein after complete capsid formation. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging (By similarity). Assembly protein plays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein UL86. Multimerizes in the nucleus such as protein UL86 forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wpo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wpo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human cytomegalovirus (HCMV) protease belongs to a new class of serine proteases, with a unique polypeptide backbone fold. The crystal structure of the protease in complex with a peptidomimetic inhibitor (based on the natural substrates and covering the P4 to P1' positions) has been determined at 2.7 A resolution. The inhibitor is bound in an extended conformation, forming an anti-parallel beta-sheet with the protease. The P3 and P1 side chains are less accessible to solvent, whereas the P4 and P2 side chains are more exposed. The inhibitor binding mode shows significant similarity to those observed for peptidomimetic inhibitors or substrates of other classes of serine proteases (chymotrypsin and subtilisin). HCMV protease therefore represents example of convergent evolution. In addition, large conformational differences relative to the structure of the free enzyme are observed, which may be important for inhibitor binding.
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===HCMV protease inhibitor complex===
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Conserved mode of peptidomimetic inhibition and substrate recognition of human cytomegalovirus protease.,Tong L, Qian C, Massariol MJ, Deziel R, Yoakim C, Lagace L Nat Struct Biol. 1998 Sep;5(9):819-26. PMID:9731777<ref>PMID:9731777</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wpo" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9731777}}, adds the Publication Abstract to the page
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*[[Virus protease 3D structures|Virus protease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9731777 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9731777}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human betaherpesvirus 5]]
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2WPO is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Human_herpesvirus_5 Human herpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WPO OCA].
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[[Category: Large Structures]]
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[[Category: Deziel R]]
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==Reference==
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[[Category: Lagace L]]
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<ref group="xtra">PMID:9731777</ref><references group="xtra"/>
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[[Category: Massariol M-J]]
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[[Category: Human herpesvirus 5]]
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[[Category: Qian C]]
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[[Category: Deziel, R.]]
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[[Category: Tong L]]
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[[Category: Lagace, L.]]
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[[Category: Yoakim C]]
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[[Category: Massariol, M J.]]
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[[Category: Qian, C.]]
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[[Category: Tong, L.]]
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[[Category: Yoakim, C.]]
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[[Category: Coat protein]]
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[[Category: Hydrolase]]
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[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 4 12:12:05 2009''
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Current revision

HCMV protease inhibitor complex

PDB ID 2wpo

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