1xvk

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(New page: 200px<br /><applet load="1xvk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xvk, resolution 1.26&Aring;" /> '''X-ray strucutre of a...)
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[[Image:1xvk.gif|left|200px]]<br /><applet load="1xvk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xvk, resolution 1.26&Aring;" />
 
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'''X-ray strucutre of an Echinomycin-(GCGTACGC)2 complex'''<br />
 
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==Overview==
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==X-ray structure of an Echinomycin-(GCGTACGC)2 complex==
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The structure of the bis-intercalation complex of the depsipeptide, antibiotic echinomycin with (CGTACG)2 has been redetermined at a higher, resolution (1.4 A) and new high-resolution structures (1.1-1.5 A) are, reported for the complexes of echinomycin with (GCGTACGC)2 (at both low, and high ionic strengths) and (ACGTACGT)2. The structures show the, expected Hoogsteen pairing for the base pairs flanking the intercalating, chromophores on the outside and Watson-Crick pairing for both base pairs, enclosed by the echinomycin. In the octamer complexes but not the hexamer, complex, the echinomycin molecule, which would possess a molecular twofold, axis were it not for the thioacetal bridge, shows twofold disorder. In all, the structures the stacking of the base pairs and chromophores is extended, by intermolecular stacking. The structures provide more precise details of, the hydrogen bonding and other interactions between the bis-intercalating, antibiotics and the duplex DNA than were previously available.
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<StructureSection load='1xvk' size='340' side='right'caption='[[1xvk]], [[Resolution|resolution]] 1.26&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xvk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_echinatus Streptomyces echinatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XVK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.26&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=N2C:N,S-DIMETHYLCYSTEINE'>N2C</scene>, <scene name='pdbligand=NCY:N-METHYLCYSTEINE'>NCY</scene>, <scene name='pdbligand=QUI:2-CARBOXYQUINOXALINE'>QUI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xvk OCA], [https://pdbe.org/1xvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xvk RCSB], [https://www.ebi.ac.uk/pdbsum/1xvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xvk ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the bis-intercalation complex of the depsipeptide antibiotic echinomycin with (CGTACG)2 has been redetermined at a higher resolution (1.4 A) and new high-resolution structures (1.1-1.5 A) are reported for the complexes of echinomycin with (GCGTACGC)2 (at both low and high ionic strengths) and (ACGTACGT)2. The structures show the expected Hoogsteen pairing for the base pairs flanking the intercalating chromophores on the outside and Watson-Crick pairing for both base pairs enclosed by the echinomycin. In the octamer complexes but not the hexamer complex, the echinomycin molecule, which would possess a molecular twofold axis were it not for the thioacetal bridge, shows twofold disorder. In all the structures the stacking of the base pairs and chromophores is extended by intermolecular stacking. The structures provide more precise details of the hydrogen bonding and other interactions between the bis-intercalating antibiotics and the duplex DNA than were previously available.
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==About this Structure==
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Structures of complexes between echinomycin and duplex DNA.,Cuesta-Seijo JA, Sheldrick GM Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:15805599<ref>PMID:15805599</ref>
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1XVK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, QUI, DSN, SCC and MVA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XVK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structures of complexes between echinomycin and duplex DNA., Cuesta-Seijo JA, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):442-8. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805599 15805599]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1xvk" style="background-color:#fffaf0;"></div>
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[[Category: Cuesta-Seijo, J.A.]]
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== References ==
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[[Category: Sheldrick, G.M.]]
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<references/>
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[[Category: DSN]]
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__TOC__
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[[Category: MG]]
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</StructureSection>
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[[Category: MVA]]
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[[Category: Large Structures]]
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[[Category: QUI]]
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[[Category: Streptomyces echinatus]]
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[[Category: SCC]]
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[[Category: Cuesta-Seijo JA]]
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[[Category: bisintercalator]]
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[[Category: Sheldrick GM]]
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[[Category: hoogsteen basepairs]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:01:16 2007''
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Current revision

X-ray structure of an Echinomycin-(GCGTACGC)2 complex

PDB ID 1xvk

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