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1ljo

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(New page: 200px<br /><applet load="1ljo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ljo, resolution 1.95&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1ljo.jpg|left|200px]]<br /><applet load="1ljo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ljo, resolution 1.95&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM2) FROM ARCHAEOGLOBUS FULGIDUS AT 1.95A RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM2) FROM ARCHAEOGLOBUS FULGIDUS AT 1.95A RESOLUTION==
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Proteins of largely unknown function related to the Sm proteins present in, the core domain of eukaryotic small nuclear ribonucleoprotein particles, have recently been detected in Archaea. In contrast to eukaryotes, Archaea, contain maximally two distinct Sm-related proteins belonging to different, subfamilies, we refer to as Sm1 and Sm2. Here we report the crystal, structures of the Sm1- and Sm2-type proteins from the hyperthermophilic, euryarchaeon Archaeoglobus fulgidus (AF-Sm1 and AF-Sm2) at a resolution of, 2.5 and 1.95 A, respectively. While the AF-Sm1 protein forms a heptameric, ring structure similar to that found in other archaeal Sm1-type proteins, the AF-Sm2 protein unexpectedly forms a homo-hexamer in the crystals, and, as is evident from the mass spectrometric analysis, also in solution. Both, proteins have essentially the same monomer fold and inter-subunit, beta-sheet hydrogen bonding giving rise to a similar overall architecture, of the doughnut-shaped six and seven-membered rings. In addition, a, conserved uracil-binding pocket identified previously in an AF-Sm1/RNA, complex, suggests a common RNA-binding mode for the AF-Sm1 and AF-Sm2, proteins, in line with solution studies showing preferential binding to, U-rich oligonucleotides for both proteins. Clear differences are however, seen in the charge distribution within the two structures. The rough faces, of the rings, i.e. the faces not containing the base binding pockets, have, opposite charges in the two structures, being predominantly positive in, AF-Sm1 and negative in AF-Sm2. Differences in the ionic interactions, between subunits provide an explanation for the distinctly different, oligomerisation behaviour of the AF-Sm1 and AF-Sm2 proteins and of Sm1-, and Sm2-type proteins in general, as well as the stability of their, complexes. Implications for the functions of archaeal Sm proteins are, being discussed.
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<StructureSection load='1ljo' size='340' side='right'caption='[[1ljo]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ljo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LJO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ljo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ljo OCA], [https://pdbe.org/1ljo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ljo RCSB], [https://www.ebi.ac.uk/pdbsum/1ljo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ljo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O29885_ARCFU O29885_ARCFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lj/1ljo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ljo ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1LJO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with CD and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LJO OCA].
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*[[Sm-like protein 3D structures|Sm-like protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Archaeal Sm proteins form heptameric and hexameric complexes: crystal structures of the Sm1 and Sm2 proteins from the hyperthermophile Archaeoglobus fulgidus., Toro I, Basquin J, Teo-Dreher H, Suck D, J Mol Biol. 2002 Jun 28;320(1):129-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12079339 12079339]
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[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Basquin, J.]]
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[[Category: Basquin J]]
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[[Category: Suck, D.]]
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[[Category: Suck D]]
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[[Category: Teo-Dreher, H.]]
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[[Category: Teo-Dreher H]]
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[[Category: Toro, I.]]
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[[Category: Toro I]]
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[[Category: ACY]]
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[[Category: CD]]
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[[Category: core snrnp domain]]
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[[Category: rna binding protein]]
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[[Category: sm]]
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[[Category: snrnp]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:02:36 2007''
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Current revision

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM2) FROM ARCHAEOGLOBUS FULGIDUS AT 1.95A RESOLUTION

PDB ID 1ljo

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