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1hua

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(New page: 200px<br /><applet load="1hua" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hua" /> '''THE SOLUTION CONFORMATION OF HYALURONAN: A C...)
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[[Image:1hua.jpg|left|200px]]<br /><applet load="1hua" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hua" />
 
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'''THE SOLUTION CONFORMATION OF HYALURONAN: A COMBINED NMR AND MOLECULAR DYNAMICS STUDY'''<br />
 
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==Overview==
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==THE SOLUTION CONFORMATION OF HYALURONAN: A COMBINED NMR AND MOLECULAR DYNAMICS STUDY==
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Hyaluronan (HA) is a negatively charged glycosaminoglycan that exhibits a, wide variety of biological effects mediated by binding to cell-surface and, extracellular matrix proteins (hyaladherins). Short HA oligosaccharides, have been shown to retain the specific interactions and biological effects, of high molecular weight HA. Although it has a simple disaccharide, repeating unit, the aqueous solution conformation of HA has been very, difficult to determine because of strong coupling and overlapping, resonances. In this study, we propose aqueous solution conformations for, an octasaccharide of HA, derived from proton-proton NOE data and, restrained molecular dynamics. To overcome spectral overlap and strong, coupling, alternate methods for extracting distance restraints were, employed. Restrained molecular dynamics calculations yielded one set of, interglycosidic angle values for the beta (1,3) linkage (phi 13 = 46, degrees, psi 13 = 24 degrees). In contrast, two sets of values for the, beta (1,4) linkage were consistent with the NOE restraints (phi 14 = 24, degrees, psi 14 = -53 degrees or phi 14 = 48 degrees, psi 14 = 8 degrees)., The potential difference in flexibility for the two linkages is consistent, with unrestrained as well as the restrained molecular dynamics, trajectories described here. The conformational parameters obtained from, restrained molecular dynamics are used to predict helical parameters of, high molecular weight HA and will provide a basis for studies of HA, binding to proteins.
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<StructureSection load='1hua' size='340' side='right'caption='[[1hua]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HUA FirstGlance]. <br>
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1HUA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HUA OCA].
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hua OCA], [https://pdbe.org/1hua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hua RCSB], [https://www.ebi.ac.uk/pdbsum/1hua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hua ProSAT]</span></td></tr>
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</table>
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==Reference==
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__TOC__
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The solution conformation of hyaluronan: a combined NMR and molecular dynamics study., Holmbeck SM, Petillo PA, Lerner LE, Biochemistry. 1994 Nov 29;33(47):14246-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7947836 7947836]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Holmbeck, S.M.A.]]
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[[Category: Holmbeck SMA]]
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[[Category: Lerner, L.E.]]
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[[Category: Lerner LE]]
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[[Category: Petillo, P.A.]]
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[[Category: Petillo PA]]
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[[Category: texture of connective tissue]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:05:39 2007''
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Current revision

THE SOLUTION CONFORMATION OF HYALURONAN: A COMBINED NMR AND MOLECULAR DYNAMICS STUDY

PDB ID 1hua

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