3eu1

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{{Seed}}
 
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[[Image:3eu1.jpg|left|200px]]
 
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==Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution==
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The line below this paragraph, containing "STRUCTURE_3eu1", creates the "Structure Box" on the page.
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<StructureSection load='3eu1' size='340' side='right'caption='[[3eu1]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3eu1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EU1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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{{STRUCTURE_3eu1| PDB=3eu1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eu1 OCA], [https://pdbe.org/3eu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eu1 RCSB], [https://www.ebi.ac.uk/pdbsum/3eu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eu1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HBA1_CAPHI HBA1_CAPHI] Involved in oxygen transport from the lung to the various peripheral tissues. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.[UniProtKB:P01946]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/3eu1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eu1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hemoglobin is a vital protein present in almost all higher species. It is a transport protein involved in carrying oxygen from lungs to tissues and carbon dioxide back to lungs by an intrinsically coordinated manner. Even though a good amount of work has been carried out in this direction there exists scarcity of structural insight on low oxygen affinity species. Attempts are being made to unravel the structural insight of this low oxygen affinity species. Goat blood plasma was collected, treated with EDTA to avoid blood clotting and purification was accomplished using DEAE-anion chromatographic column. The goat hemoglobin was crystallized using 50mM of phosphate buffer at pH 6.7 with 1M NaCl and PEG 3350 as precipitant by hanging drop vapor diffusion method. Crystals obtained are screened and suitable crystals are taken for data collection using mar345dtb as image plate detector system. Goat hemoglobin crystal diffracted up to 2.61 A resolution. Goat hemoglobin crystallizes in orthorhombic space group P212(1)2(1) as a whole biological molecule in the asymmetric unit with cell dimensions a=53.568A, b=67.365A, c=154.183A.
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===Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution===
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Purification, Crystallization and Preliminary X-Ray Diffraction Studies on Goat (Capra hircus) Hemoglobin - A Low Oxygen Affinity Species.,Moorthy PS, Neelagandan K, Balasubramanian M, Ponnuswamy MN Protein Pept Lett. 2009;16(4):454-6. PMID:19356147<ref>PMID:19356147</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3eu1" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19356147}}, adds the Publication Abstract to the page
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19356147 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19356147}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3EU1 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EU1 OCA].
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==Reference==
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<ref group="xtra">PMID:19356147</ref><references group="xtra"/>
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[[Category: Capra hircus]]
[[Category: Capra hircus]]
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[[Category: Balasubramanian, M.]]
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[[Category: Large Structures]]
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[[Category: Moorthy, Pon Sathya.]]
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[[Category: Balasubramanian M]]
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[[Category: Neelagandan, K.]]
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[[Category: Neelagandan K]]
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[[Category: Ponnuswamy, M N.]]
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[[Category: Ponnuswamy MN]]
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[[Category: 3-diphospho glycerate]]
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[[Category: Sathya Moorthy P]]
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[[Category: Allosteric mechanism]]
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[[Category: Capra hircus]]
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[[Category: Hemoglobin]]
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[[Category: Low oxygen affinity]]
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[[Category: Oxygen storage]]
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[[Category: Oxygen transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 11 22:18:49 2009''
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Current revision

Crystal Structure determination of goat hemoglobin (Capra hircus) at 3 angstrom resolution

PDB ID 3eu1

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