2fft

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{{Seed}}
 
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[[Image:2fft.png|left|200px]]
 
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==NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles==
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The line below this paragraph, containing "STRUCTURE_2fft", creates the "Structure Box" on the page.
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<StructureSection load='2fft' size='340' side='right'caption='[[2fft]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FFT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fft OCA], [https://pdbe.org/2fft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fft RCSB], [https://www.ebi.ac.uk/pdbsum/2fft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fft ProSAT], [https://www.topsan.org/Proteins/CESG/2fft TOPSAN]</span></td></tr>
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{{STRUCTURE_2fft| PDB=2fft | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GT36_SPIOL Q8GT36_SPIOL]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Thylakoid soluble phosphoprotein of 9 kDa (TSP9) has been identified as a plant-specific protein in the photosynthetic thylakoid membrane (Carlberg et al. (2003) Proc. Natl. Acad. Sci. 100, 757-762). Nonphosphorylated TSP9 is associated with the membrane, whereas, after light-induced phosphorylation, a fraction of the phosphorylated TSP9 is released into the aqueous stroma. By NMR spectroscopy, we have determined the structural features of nonphosphorylated TSP9 both in aqueous solution and in membrane mimetic micelles. The results show that both wild type nonphosphorylated TSP9 and a triple-mutant (T46E + T53E + T60E) mimic of the triphosphorylated form of TSP9 are disordered under aqueous conditions, but adopt an ordered conformation in the presence of detergent micelles. The micelle-induced structural features, which are similar in micelles either of SDS or dodecylphosphocholine (DPC), consist of an N-terminal alpha-helix, which may represent the primary site of interaction between TSP9 and binding partners, and a less structured helical turn near the C-terminus. These structured elements contain mainly hydrophobic residues. NMR relaxation data for nonphosphorylated TSP9 in SDS micelles indicated that the molecule is highly flexible with the highest order in the N-terminal alpha-helix. Intermolecular NOE signals, as well as spin probe-induced broadening of NMR signals, demonstrated that the SDS micelles contact both the structured and a portion of the unstructured regions of TSP9, in particular, those containing the three phosphorylation sites (T46, T53, and T60). This interaction may explain the selective dissociation of phosphorylated TSP9 from the membrane. Our study presents a structural model for the role played by the structured and unstructured regions of TSP9 in its membrane association and biological function.
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===NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles===
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Micelle-induced folding of spinach thylakoid soluble phosphoprotein of 9 kDa and its functional implications.,Song J, Lee MS, Carlberg I, Vener AV, Markley JL Biochemistry. 2006 Dec 26;45(51):15633-43. Epub 2006 Dec 8. PMID:17176085<ref>PMID:17176085</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_17176085}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2fft" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17176085 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17176085}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2FFT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFT OCA].
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==Reference==
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<ref group="xtra">PMID:17176085</ref><references group="xtra"/>
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[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
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[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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[[Category: Carlberg I]]
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[[Category: Carlberg, I.]]
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[[Category: Lee MS]]
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[[Category: Lee, M S.]]
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[[Category: Markley JL]]
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[[Category: Markley, J L.]]
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[[Category: Song J]]
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[[Category: Song, J.]]
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[[Category: Center for eukaryotic structural genomic]]
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[[Category: Cesg]]
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[[Category: Plant protein]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Thylakoid soluble phosphoprotein]]
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[[Category: Tsp9]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 11 22:43:09 2009''
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Current revision

NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles

PDB ID 2fft

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